PE/Cy7® Anti-F4/80 antibody [BM8.1] (ab218761)

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Flow Cytometry - PE/Cy7® Anti-F4/80 antibody [BM8.1] (ab218761)

    Key features and details

    • PE/Cy7® Rat monoclonal [BM8.1] to F4/80
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
    • Isotype: IgG2b

    Overview

    • Product name

      PE/Cy7® Anti-F4/80 antibody [BM8.1]
      See all F4/80 primary antibodies

    • Description

      PE/Cy7® Rat monoclonal [BM8.1] to F4/80

    • Host species

      Rat

    • Conjugation

      PE/Cy7®. Ex: 496nm, Em: 774nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Positive control

      • C57Bl/6 bone marrow cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
    • Concentration information loading...

    • Purity

      Protein A purified

    • Purification notes

      ab218761 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    • Clonality

      Monoclonal

    • Clone number

      BM8.1

    • Isotype

      IgG2b

    • Light chain type

      kappa

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab218761 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    Target

    • Function

      Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

    • Tissue specificity

      Expression is restricted to eosinophils.

    • Sequence similarities

      Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
      Contains 6 EGF-like domains.
      Contains 1 GPS domain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ADGRE1 antibody
        • Adhesion G protein coupled receptor E1 antibody
        • Adhesion G protein-coupled receptor E1 antibody
        • AGRE1_HUMAN antibody
        • Cell surface glycoprotein EMR1 antibody
        • Cell surface glycoprotein F4/80 antibody
        • DD7A5 7 antibody
        • Egf like module containing mucin like hormone receptor like 1 antibody
        • Egf like module containing mucin like hormone receptor like sequence 1 antibody
        • EGF like module receptor 1 antibody
        • EGF TM7 antibody
        • EGF-like module receptor 1 antibody
        • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
        • EGFTM7 antibody
        • EMR 1 antibody
        • EMR1 antibody
        • EMR1 hormone receptor antibody
        • Gpf480 antibody
        • Ly71 antibody
        • Lymphocyte antigen 71 antibody
        • TM7LN3 antibody
        see all

      Images

      • Flow Cytometry - PE/Cy7® Anti-F4/80 antibody [BM8.1] (ab218761)
        Flow Cytometry - PE/Cy7® Anti-F4/80 antibody [BM8.1] (ab218761)

        Flow Cytometric analysis of C57Bl/6 bone marrow cells labeling F4/80 with APC Anti-Mouse CD11b and 0.25 µg ab218761 (right panel) or 0.25 µg PE-Cyanine7 Rat IgG2b isotype control (left panel).

      References (0)

      Publishing research using ab218761? Please let us know so that we can cite the reference in this datasheet.

      ab218761 has not yet been referenced specifically in any publications.

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