PE/Cy7® Anti-FAS Intracellular Fragment antibody [LT95] (ab234275)
![Flow Cytometry - PE/Cy7® Anti-FAS Intracellular Fragment antibody [LT95] (ab234275)](https://www.abcam.com/ps/products/234/ab234275/Images/ab234275-308331-2342751.jpg "Flow Cytometry - PE/Cy7® Anti-FAS Intracellular Fragment antibody [LT95] (ab234275)")
Key features and details
- PE/Cy7® Mouse monoclonal [LT95] to FAS Intracellular Fragment
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG1
Overview
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Product name
PE/Cy7® Anti-FAS Intracellular Fragment antibody [LT95] -
Description
PE/Cy7® Mouse monoclonal [LT95] to FAS Intracellular Fragment -
Host species
Mouse -
Conjugation
PE/Cy7®. Ex: 496nm, Em: 774nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to FAS Intracellular Fragment. (HUT-78 human T cell lymphoma cell line)
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Positive control
- Flow Cytometry: Human peripheral blood.
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
ab234275 is conjugated with tandem dye PE-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
LT95 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab234275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 4µl for 106 cells. |
Target
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Relevance
Fas is a receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Isoform 1 is located at the cell membrane as a single-pass type I meembrane protein. -
Cellular localization
Cell Membrane; Single-pass type I membrane protein. -
Database links
- Entrez Gene: 355 Human
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Alternative names
- Apoptosis mediating surface antigen FAS antibody
- APT1 antibody
- TNF receptor superfamily member 6 antibody
- Tumor necrosis factor receptor superfamily member 6 antibody
Images
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Flow Cytometry - PE/Cy7® Anti-FAS Intracellular Fragment antibody [LT95] (ab234275)
Flow Cytometry analysis of human peripheral blood labeling FAS Intracellular Fragment with ab234275. Surface staining. Gated on leukocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab234275 has not yet been referenced specifically in any publications.
