PE/Cy7® Anti-NKG2D antibody [CX5] (ab95796)

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Flow Cytometry - PE/Cy7® Anti-NKG2D antibody [CX5] (ab95796)

    Key features and details

    • PE/Cy7® Rat monoclonal [CX5] to NKG2D
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
    • Isotype: IgG1

    Overview

    • Product name

      PE/Cy7® Anti-NKG2D antibody [CX5]
      See all NKG2D primary antibodies

    • Description

      PE/Cy7® Rat monoclonal [CX5] to NKG2D

    • Host species

      Rat

    • Conjugation

      PE/Cy7®. Ex: 496nm, Em: 774nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Full length Mouse protein

    • Positive control

      • Splenocytes

    • General notes

      This tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage and all handling steps. When staining cells with PE-Cy7-conjugated antibodies, always protect samples from light.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituent: PBS

      Protein stabiliser
    • Concentration information loading...

    • Purity

      Protein G purified

    • Clonality

      Monoclonal

    • Clone number

      CX5

    • Isotype

      IgG1

    • Light chain type

      kappa

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab95796 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.25µg for 105-8 cells.

    Perform staining in a final volume of 100µl.

    Target

    • Function

      Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.

    • Tissue specificity

      Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).

    • Sequence similarities

      Contains 1 C-type lectin domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD314 antibody
        • CD314 antigen antibody
        • D12S2489E antibody
        • Killer cell lectin like receptor subfamily K member 1 antibody
        • Killer cell lectin-like receptor subfamily K member 1 antibody
        • KLR antibody
        • KLRC4 KLRK1 readthrough antibody
        • KLRK1 antibody
        • NK cell receptor D antibody
        • NK lectin-like receptor antibody
        • NKG2 D activating NK receptor antibody
        • NKG2 D type II integral membrane protein antibody
        • NKG2-D antibody
        • NKG2-D type II integral membrane protein antibody
        • NKG2-D-activating NK receptor antibody
        • Nkg2d antibody
        • NKG2D_HUMAN antibody
        • NKLLR antibody
        • NKR P2 antibody
        • Nkrp2 antibody
        see all

      Images

      • Flow Cytometry - PE/Cy7® Anti-NKG2D antibody [CX5] (ab95796)
        Flow Cytometry - PE/Cy7® Anti-NKG2D antibody [CX5] (ab95796)
        Staining of C57BL/6 splenocytes with anti-CD49b FITC and either 0.125µg of Rat IgG1k isotype control PE-Cy7 (left) or 0.125µg of ab95796 (right).
        Cells in the lymphocyte gate were used for analysis.

      References (0)

      Publishing research using ab95796? Please let us know so that we can cite the reference in this datasheet.

      ab95796 has not yet been referenced specifically in any publications.

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