PE/Cy7® Mouse IgG1 kappa Isotype Control (ab239464)
Overview
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Product name
PE/Cy7® Mouse IgG1 kappa Isotype Control -
Conjugation
PE/Cy7®. Ex: 496nm, Em: 774nm -
Specificity
ab239464 has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
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General notes
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibody is conjugated with tandem dye PE/Cy7 ® under optimum conditions. The reagent is free of unconjugated PE/Cy7 ®. -
Clonality
Monoclonal -
Clone number
MOPC-21 -
Isotype
IgG1 -
Light chain type
kappa
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab239464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab239464 is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background. |
Images
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Flow Cytometry - PE/Cy7® Mouse IgG1 kappa Isotype Control (ab239464)
Example of non-specific ab239464 signal on human peripheral blood; surface staining, 0.33 μg/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab239464 has not yet been referenced specifically in any publications.
