• Product name

  • Description

    Rabbit polyclonal to PEDF
  • Host species

  • Specificity

    Recognizes PEDF at 50kDa. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein (His-tag) corresponding to Human PEDF. Corresponding to full length human PEDF expressed in baby hamster kidney cells.

  • Positive control

    • WB: RIPA lysates from Y79 cells.
  • General notes

    For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.



Our Abpromise guarantee covers the use of ab14993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


  • Function

    Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificity

    Retinal pigment epithelial cells and blood plasma.
  • Sequence similarities

    Belongs to the serpin family.
  • Developmental stage

    Expressed in quiescent cells.
  • Domain

    The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
  • Post-translational

    The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization

    Secreted. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation-inducing gene 35 protein antibody
    • EPC 1 antibody
    • EPC-1 antibody
    • EPC1 antibody
    • OI12 antibody
    • OI6 antibody
    • PEDF antibody
    • PEDF_HUMAN antibody
    • PIG 35 antibody
    • PIG35 antibody
    • Pigment epithelium derived factor antibody
    • Pigment epithelium-derived factor antibody
    • Proliferation inducing protein 35 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • Serpin F1 antibody
    • Serpin family F member 1 antibody
    • Serpin peptidase inhibitor antibody
    • Serpin peptidase inhibitor clade F member 1 antibody
    • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • SERPINF 1 antibody
    • Serpinf1 antibody
    see all


  • Lanes 1-4: Cartilage lysates from 4 osteoarthritic knee replacement donors (100 mg (wet weight) powdered cartilage/ 1 ml RIPA buffer, extracted for 24 hrs at 4°C)

    Lane C: Positive control (overexpressing cell line)

    Anticipated band at approx 50kDa detected. Specificity of antibody allowed relatively long exposure and quite high concentrations of primary and secondary antibodies to be used with negligible background. Good results were also obtained with unreduced samples.
    The additional approx 250kDa band is likely to be due to PEDF crosslinked or bound to high molecular weight matrix proteins (collagen, aggrecan) which are present in cartilage lysates. Reduction using beta mercaptoethanol reduced some, but not all high MW staining and increased the intensity of the 50kDa band.

  • Anti-PEDF antibody (ab14993) at 1 µg/ml + Y79 (Human retinoblastoma cell line) cell lysate in RIPA buffer at 40 µg

    IgG (HRP) goat anti-rabbit

    Predicted band size: 50 kDa

    Chemiluminescence detection system.


This product has been referenced in:

  • Qian W  et al. Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Mol Med Rep 17:6515-6525 (2018). WB . Read more (PubMed: 29532868) »
  • Han Z  et al. Retinal angiogenesis in the Ins2(Akita) mouse model of diabetic retinopathy. Invest Ophthalmol Vis Sci 54:574-84 (2013). WB . Read more (PubMed: 23221078) »
See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


I am very pleased to hear you would like to accept our offer and test ab14993, ab30788 ,ab64053 and ab115489 in sELISA. These codes will give you: 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please submit an Abreview for sELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Thank you very much for your interest in PEDF and Somatostatin antibodies.

To our knowledge,none of our antibodies have been tested in sELISA. However, I have picked out four antibodies, which I found most suitable for this purpose:


https://www.abcam.com/index.html?datasheet=14993 (or use the following: https://www.abcam.com/index.html?datasheet=14993).

https://www.abcam.com/index.html?datasheet=115489 (or use the following: https://www.abcam.com/index.html?datasheet=115489).


https://www.abcam.com/index.html?datasheet=64053 (or use the following: https://www.abcam.com/index.html?datasheet=64053).

https://www.abcam.com/index.html?datasheet=30788 (or use the following: https://www.abcam.com/index.html?datasheet=30788).

I can offer a discount off a future purchase if you buyone or bothof these antibody couplesnow, test it insELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody per antibody ordered.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab14993, ab30788, ab64053 andab115489 in sELISA. I will then send a discount code. This code must be issued before purchasing ab14993, ab30788, ab64053 and ab115489 so please wait for my reply before ordering.

2. Purchase ab14993, ab30788, ab64053 and ab115489 either by phone, fax, or online (www.abcam.com).

3. Test it in sELISA.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab14993, ab30788, ab64053 and ab115489 turns out to be unsuitable for sELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Thank you for contacting us.

Here are the links to the databases that could help identify potential tissue or cell types that might not express the PEDF protein.

UniProt: http://www.uniprot.org/uniprot/P36955
Human Protein Atlas: http://www.proteinatlas.org/ENSG00000132386

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Western blot
Human Tissue lysate - whole (Cartilage)
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. John Wardale

Verified customer

Submitted Sep 24 2008

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