Anti-PEDF antibody (ab14993)
Key features and details
- Rabbit polyclonal to PEDF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-PEDF antibody
See all PEDF primary antibodies -
Description
Rabbit polyclonal to PEDF -
Host species
Rabbit -
Specificity
Recognizes PEDF at 50kDa. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Human PEDF. Expressed in baby hamster kidney cells.
Database link: P36955 -
Positive control
- WB: RIPA lysates from Y79 cells.
-
General notes
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
|
Notes |
---|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
Target
-
Function
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
Tissue specificity
Retinal pigment epithelial cells and blood plasma. -
Sequence similarities
Belongs to the serpin family. -
Developmental stage
Expressed in quiescent cells. -
Domain
The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. -
Post-translational
modificationsThe N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
-
Database links
- Entrez Gene: 5176 Human
- Omim: 172860 Human
- SwissProt: P36955 Human
- Unigene: 532768 Human
-
Alternative names
- Cell proliferation-inducing gene 35 protein antibody
- EPC 1 antibody
- EPC-1 antibody
see all
Images
-
Lanes 1-4: Cartilage lysates from 4 osteoarthritic knee replacement donors (100 mg (wet weight) powdered cartilage/ 1 ml RIPA buffer, extracted for 24 hrs at 4°C)
Lane C: Positive control (overexpressing cell line)
Anticipated band at approx 50kDa detected. Specificity of antibody allowed relatively long exposure and quite high concentrations of primary and secondary antibodies to be used with negligible background. Good results were also obtained with unreduced samples.
The additional approx 250kDa band is likely to be due to PEDF crosslinked or bound to high molecular weight matrix proteins (collagen, aggrecan) which are present in cartilage lysates. Reduction using beta mercaptoethanol reduced some, but not all high MW staining and increased the intensity of the 50kDa band. -
Anti-PEDF antibody (ab14993) at 1 µg/ml + Y79 (Human retinoblastoma cell line) cell lysate in RIPA buffer at 40 µg
Secondary
IgG (HRP) goat anti-rabbit
Predicted band size: 50 kDaChemiluminescence detection system.
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab14993 has been referenced in 9 publications.
- Sun X et al. Improvement of the bladder perfusion curative effect through tight junction protein degradation induced by magnetic temperature-sensitive hydrogels. Front Bioeng Biotechnol 10:958072 (2022). PubMed: 35992356
- Li B et al. lncRNA GABPB1 intronic transcript 1 upregulates pigment epithelium-derived factor via miR-93 to suppress cell proliferation in hepatocellular carcinoma. Oncol Lett 21:260 (2021). PubMed: 33664823
- Zhang X et al. Pigment epithelium-derived factor facilitates NLRP3 inflammasome activation through downregulating cytidine monophosphate kinase 2: A potential treatment strategy for missed abortion. Int J Mol Med 45:1436-1446 (2020). PubMed: 32323732
- Qian W et al. Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Mol Med Rep 17:6515-6525 (2018). WB . PubMed: 29532868
- Han Z et al. Retinal angiogenesis in the Ins2(Akita) mouse model of diabetic retinopathy. Invest Ophthalmol Vis Sci 54:574-84 (2013). WB . PubMed: 23221078
- Allen CL et al. Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing. PLoS One 8:e78441 (2013). ICC/IF . PubMed: 24205233
- Tripathy D et al. Age-related decrease in cerebrovascular-derived neuroprotective proteins: effect of acetaminophen. Microvasc Res 84:278-85 (2012). ELISA ; Rat . PubMed: 22944728
- Sanchez A et al. Pigment epithelium-derived factor (PEDF) protects cortical neurons in vitro from oxidant injury by activation of extracellular signal-regulated kinase (ERK) 1/2 and induction of Bcl-2. Neurosci Res 72:1-8 (2012). ELISA . PubMed: 21946416
- Simper D et al. Comparative proteomics profiling reveals role of smooth muscle progenitors in extracellular matrix production. Arterioscler Thromb Vasc Biol 30:1325-32 (2010). WB ; Human . PubMed: 20431068