Key features and details
- Rabbit polyclonal to PEDF
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-PEDF antibody
See all PEDF primary antibodies
DescriptionRabbit polyclonal to PEDF
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
- IHC-P: Rat brain tissue and rat eye ciliary body.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200.
FunctionNeurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Tissue specificityRetinal pigment epithelial cells and blood plasma.
Sequence similaritiesBelongs to the serpin family.
Developmental stageExpressed in quiescent cells.
DomainThe N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
modificationsThe N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localizationSecreted. Melanosome. Enriched in stage I melanosomes.
- Information by UniProt
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat eye ciliary body tissue labeling PEDF using ab203033 at a 1/200 dilution, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution (40 mins at 37°C).
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat brain tissue labeling PEDF using ab203033 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab203033 has not yet been referenced specifically in any publications.