Anti-PEDF antibody (ab227295)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PEDF antibody
See all PEDF primary antibodies -
Description
Rabbit polyclonal to PEDF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human PEDF (internal sequence). The exact sequence is proprietary.
Database link: P36955 -
Positive control
- WB: HepG2 whole cell lysate. IHC-P: Mouse eye tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 46 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
Tissue specificity
Retinal pigment epithelial cells and blood plasma. -
Sequence similarities
Belongs to the serpin family. -
Developmental stage
Expressed in quiescent cells. -
Domain
The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. -
Post-translational
modificationsThe N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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Database links
- Entrez Gene: 5176 Human
- Entrez Gene: 20317 Mouse
- Omim: 172860 Human
- SwissProt: P36955 Human
- SwissProt: P97298 Mouse
- Unigene: 532768 Human
- Unigene: 2044 Mouse
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Alternative names
- Cell proliferation-inducing gene 35 protein antibody
- EPC 1 antibody
- EPC-1 antibody
see all
Images
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Western blot - Anti-PEDF antibody (ab227295)Anti-PEDF antibody (ab227295) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 46 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEDF antibody (ab227295)Paraffin-embedded mouse eye tissue stained for PEDF using ab227295 at 1/400 dilution in immunohistochemical analysis.
Antigen retrieval: Citrate buffer pH 6.0 for 15 mins.
References
ab227295 has not yet been referenced specifically in any publications.
