Key features and details
- Rabbit polyclonal to PEDF
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PEDF antibody
See all PEDF primary antibodies
DescriptionRabbit polyclonal to PEDF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human brain and glioma tissue. WB: Recombinant human PEDF protein; Human serum.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 46 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionNeurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Tissue specificityRetinal pigment epithelial cells and blood plasma.
Sequence similaritiesBelongs to the serpin family.
Developmental stageExpressed in quiescent cells.
DomainThe N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
modificationsThe N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localizationSecreted. Melanosome. Enriched in stage I melanosomes.
- Information by UniProt
- Cell proliferation-inducing gene 35 protein antibody
- EPC 1 antibody
- EPC-1 antibody
Anti-PEDF antibody (ab233120) at 3 µg/ml + Human serum
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Anti-PEDF antibody (ab233120) at 2 µg/ml + Recombinant human PEDF protein
Predicted band size: 46 kDa
Formalin-fixed, paraffin-embedded human glioma tissue stained for PEDF using ab233120 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human brain tissue stained for PEDF using ab233120 at 20 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233120 has been referenced in 1 publication.
- Tao YJ et al. Pirfenidone suppresses the abnormal activation of human Müller cells after platelet-derived growth factor-BB stimulation. Int J Ophthalmol 12:1075-1082 (2019). PubMed: 31341795