Key features and details
- Rabbit polyclonal to PEDF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PEDF antibody
See all PEDF primary antibodies
DescriptionRabbit polyclonal to PEDF
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide within Human PEDF aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available as
- WB: Plasma total protein lysate. Human kidney tissue lysate - total protein.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46 kDa).|
FunctionNeurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Tissue specificityRetinal pigment epithelial cells and blood plasma.
Sequence similaritiesBelongs to the serpin family.
Developmental stageExpressed in quiescent cells.
DomainThe N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
modificationsThe N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localizationSecreted. Melanosome. Enriched in stage I melanosomes.
- Information by UniProt
- Cell proliferation-inducing gene 35 protein antibody
- EPC 1 antibody
- EPC-1 antibody
All lanes : Anti-PEDF antibody (ab74439) at 1 µg/ml
Lane 1 : Human plasma total protein lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Lane 1 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lane 2 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
PEDF is known to be phosphorylated and glycosylated. This could explain the bands appearing higher than the expected molecular weight.
ab74439 has not yet been referenced specifically in any publications.