Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to PEDF - BSA and Azide free (Detector)
- Suitable for: Sandwich ELISA
- Reacts with: Human
Product nameAnti-PEDF antibody - BSA and Azide free (Detector)
See all PEDF primary antibodies
DescriptionRabbit monoclonal to PEDF - BSA and Azide free (Detector)
Tested applicationsSuitable for: Sandwich ELISAmore details
Species reactivityReacts with: Human
ab259608 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PEDF. The recommended pair for sandwich ELISA is:
Capture: ab259607, Human PEDF Capture Antibody (unconjugated)
Detector: ab259608, Human PEDF Detector Antibody (unconjugated)
The reference range value is 468.75 - 30000 pg/ml.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
-High batch-to-batch consistency and reproducibility
-Improved sensitivity and specificity
-Long-term security of supply
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C.
Concentration information loading...
sELISA pair antibody
Our Abpromise guarantee covers the use of ab259608 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal to PEDF - BSA and Azide free (Capture) (ab259607).|
FunctionNeurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Tissue specificityRetinal pigment epithelial cells and blood plasma.
Sequence similaritiesBelongs to the serpin family.
Developmental stageExpressed in quiescent cells.
DomainThe N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
modificationsThe N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localizationSecreted. Melanosome. Enriched in stage I melanosomes.
- Information by UniProt
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ab259608 has not yet been referenced specifically in any publications.