Overview

  • Product name

    Anti-PEDF antibody [EPR9826(B)]
    See all PEDF primary antibodies
  • Description

    Rabbit monoclonal [EPR9826(B)] to PEDF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PEDF aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab168543)

  • Positive control

    • WB: Human plasma; Human serum; Y79, A549, HEK-293T and K562 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.Can be blocked with PEDF peptide (ab168543).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
    • Tissue specificity

      Retinal pigment epithelial cells and blood plasma.
    • Sequence similarities

      Belongs to the serpin family.
    • Developmental stage

      Expressed in quiescent cells.
    • Domain

      The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
    • Post-translational
      modifications

      The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
      N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • Cellular localization

      Secreted. Melanosome. Enriched in stage I melanosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cell proliferation-inducing gene 35 protein antibody
      • EPC 1 antibody
      • EPC-1 antibody
      • EPC1 antibody
      • OI12 antibody
      • OI6 antibody
      • PEDF antibody
      • PEDF_HUMAN antibody
      • PIG 35 antibody
      • PIG35 antibody
      • Pigment epithelium derived factor antibody
      • Pigment epithelium-derived factor antibody
      • Proliferation inducing protein 35 antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
      • Serpin F1 antibody
      • Serpin family F member 1 antibody
      • Serpin peptidase inhibitor antibody
      • Serpin peptidase inhibitor clade F member 1 antibody
      • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
      • SERPINF 1 antibody
      • Serpinf1 antibody
      see all

    Images

    • All lanes : Anti-PEDF antibody [EPR9826(B)] (ab157207) at 1/1000 dilution

      Lane 1 : Human plasma
      Lane 2 : Human serum
      Lane 3 : Y79 (Human retinoblastoma cell line) lysate
      Lane 4 : A549 (Human lung carcinoma cell line) lysate
      Lane 5 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
      Lane 6 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 46 kDa

    References

    This product has been referenced in:

    • Zhang R  et al. A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. Nature 535:164-8 (2016). Read more (PubMed: 27383988) »
    • Chen X  et al. Metformin inhibits prostate cancer cell proliferation, migration, and tumor growth through upregulation of PEDF expression. Cancer Biol Ther 17:507-14 (2016). Read more (PubMed: 26987032) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157207.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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