Overview

  • Product name
    Anti-PEDF antibody [EPR9826(B)]
    See all PEDF primary antibodies
  • Description
    Rabbit monoclonal [EPR9826(B)] to PEDF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PEDF aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab168543)

  • Positive control
    • Human plasma; Human serum; Y79, A549, 293T and K562 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.Can be blocked with PEDF peptide (ab168543).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
    • Tissue specificity
      Retinal pigment epithelial cells and blood plasma.
    • Sequence similarities
      Belongs to the serpin family.
    • Developmental stage
      Expressed in quiescent cells.
    • Domain
      The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
    • Post-translational
      modifications
      The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
      N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • Cellular localization
      Secreted. Melanosome. Enriched in stage I melanosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell proliferation-inducing gene 35 protein antibody
      • EPC 1 antibody
      • EPC-1 antibody
      • EPC1 antibody
      • OI12 antibody
      • OI6 antibody
      • PEDF antibody
      • PEDF_HUMAN antibody
      • PIG 35 antibody
      • PIG35 antibody
      • Pigment epithelium derived factor antibody
      • Pigment epithelium-derived factor antibody
      • Proliferation inducing protein 35 antibody
      • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
      • Serpin F1 antibody
      • Serpin family F member 1 antibody
      • Serpin peptidase inhibitor clade F member 1 antibody
      • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
      • SERPINF 1 antibody
      • Serpinf1 antibody
      see all

    Images

    • All lanes : Anti-PEDF antibody [EPR9826(B)] (ab157207) at 1/1000 dilution

      Lane 1 : Human plasma
      Lane 2 : Human serum
      Lane 3 : Y79 lysate
      Lane 4 : A549 lysate
      Lane 5 : 293T lysate
      Lane 6 : K562 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 46 kDa

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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