PE/DyLight™ 594 Anti-CD45RA antibody [MEM-56] (ab239324)
![Flow Cytometry - PE/DyLight™ 594 Anti-CD45RA antibody [MEM-56] (ab239324)](https://www.abcam.com/ps/products/239/ab239324/Images/ab239324-317631-anti-cd45ra-antibody-mem-56-pe-dylight-594-flow-cytometry.jpg "Flow Cytometry - PE/DyLight™ 594 Anti-CD45RA antibody [MEM-56] (ab239324)")
Key features and details
- PE/DyLight™ 594 Mouse monoclonal [MEM-56] to CD45RA
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/DyLight™ 594
- Isotype: IgG2b
Overview
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Product name
PE/DyLight™ 594 Anti-CD45RA antibody [MEM-56]
See all CD45RA primary antibodies -
Description
PE/DyLight™ 594 Mouse monoclonal [MEM-56] to CD45RA -
Host species
Mouse -
Conjugation
PE/DyLight™ 594 -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD45RA. Human thymocytes and T lymphocytes.
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Positive control
- Flow Cyt: Human peripheral blood.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with tandem dye PE-DyLight™ 594 (PE-DL594) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
Clonality
Monoclonal -
Clone number
MEM-56 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab239324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 4µl for 106 cells. (or 100 µl of whole blood) |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
Involvement in disease
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
Multiple sclerosis -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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Alternative names
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
Images
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Flow Cytometry - PE/DyLight™ 594 Anti-CD45RA antibody [MEM-56] (ab239324)
Flow cytometric analysis of human peripheral blood labeling CD45RA using ab239324. Surface staining. Gated on leukocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab239324 has not yet been referenced specifically in any publications.
