Product nameAnti-PEF1 antibody [EPR9310]
See all PEF1 primary antibodies
DescriptionRabbit monoclonal [EPR9310] to PEF1
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PEF1 aa 100-200. The exact sequence is proprietary.
- Jurkat, HeLa, 293T, and HL60 cell lysates; 293T cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 30 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Sequence similaritiesContains 5 EF-hand domains.
Cellular localizationCytoplasm. Membrane. Membrane-associated in the presence of C.
- Information by UniProt
- ABP32 antibody
- PEF antibody
- PEF protein with a long N terminal hydrophobic domain antibody
All lanes : Anti-PEF1 antibody [EPR9310] (ab137127) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Flow Cytometric analysis of permeabilized 293T cells labelling PEF1 with ab137127 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
ab137127 has been referenced in 1 publication.
- McGourty CA et al. Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor. Cell 167:525-538.e14 (2016). PubMed: 27716508