Overview

  • Product name
    PEG Virus Precipitation Kit
  • Sample type
    Other biological fluids, Cell culture media
  • Assay type
    Direct
  • Sensitivity
    > 40 %
  • Range
    40 % - 100 %
  • Product overview

    Abcam's PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate viruses without ultra-centrifugation. The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer. The kit can be used to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples. Viruses can be concentrated over 100 fold. An optimized Virus Re-suspension Solution is provided to maximize viral recovery by 40-100% depending on the virus type and sources. The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Viruses are usually produced at a low titer. They often need to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate viruses and remove impurities.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 50 tests
    PEG Solution 1 x 125ml
    Virus Re-suspension Solution 1 x 10ml
  • Research areas
  • Relevance
    Virus is usually produced at a low titer. It often needs to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate virus and remove impurities. PEG (Polyethylene glycol) Virus Precipitation provides an easy, convenient and time-saving method to concentrate virus without ultra-centrifugation.
  • Alternative names
    • Polyethylene glycol virus

Images

  • Functional Studies - PEG Virus Precipitation Kit (ab102538)
    Functional Studies - PEG Virus Precipitation Kit (ab102538)

    Concentration of baculovirus: Low titer baculovirus (10 ml) expressing human Granzyme B was precipitated following the kit protocol and the precipitate was suspended in 1 ml of Virus Re-suspension Solution. Both low titer and precipitated baculovirus was subsequently used to infect SF9 Insect cells. The activity of recombinant Granzyme B secreted into the culture medium by low titer and precipitated baculovirus infected insect cells was monitored using ab157403 - Granzyme B Activity Assay Kit (Fluorometric).

Protocols

References

This product has been referenced in:
  • Rotem N  et al. ALS Along the Axons - Expression of Coding and Noncoding RNA Differs in Axons of ALS models. Sci Rep 7:44500 (2017). Read more (PubMed: 28300211) »
  • Gershoni-Emek N  et al. Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1. Mol Cell Proteomics 15:506-22 (2016). Functional Studies . Read more (PubMed: 26598648) »

See all 6 Publications for this product

Publishing research using ab102538? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

11 reviews or Q&A

10
Votes: Highest First

PEG precipitation of Orthonairovirus

 Good Excellent 5/5 (Ease of Use)
Abreviews
The protocol is simple and easy to follow, without the requirement for specialist equipment or buffers for precipiation.
abreview image
Username

Abcam user community

Verified customer

Submitted May 14 2018

I am having trouble resuspending the pellet after centrifugation. Even when using a volume equal to the starting volume, the pellet does not want to go into solution. Do you have any suggestions?

You can use gentle sonication in a water bath to completely dissociate the pellet and then resuspend it in the virus resuspension solution. Make sure the sonication does not build heat in the sample.

have you tested the kit on enteroviruses / Picornaviruses? If not, any
chances it could work?

This PEG Virus Precipitation Kit (ab102538) can be used to concentrate viruses and is not specific to a particular type. Although we list retroviruses, baculoviruses, lentiviruses, and phages on our datasheet, the list does not stop there and this kit ...

Read More

1. Is there an advantage to using this product over sucrose cushion technique?
2. Will this kit work to purify virus like particles?

The advantages of this PEG Virus Precipitation Kit are:
1. Ease of use, no need for ultracentrifugation and collection of the specific sucrose density layer.
2. High viral titer recovery and concentration.
3. Wide variety of viruses can be...

Read More

I would like to know if this kit can be used to concentrate virus after employing flocculation method.

We have not tested the PEG virus precipitation kit with samples post-flocculation but assuming the product of the flocculation is an aqueous solution, the kit should be compatible with this sample. However, it is unknown how much more concentration of ...

Read More

Is the virus resuspension solution compatible with EM?

If the presence of glycerol or salts is not going to work for your specific EM experiment that you can resuspend the virus pellet in your own buffer of choice.

Dear Sir,

This is all very well. However, I'm not looking for an antibody.

I would like to do a test with the PEG Virus Precipitation Kit (ab102538).

Sincerely,

Thank you for your reply and my sincere apologies for my confusion.

I am sorry we are regrettably not able to provide samples for testing, as discussed in the previous email. However, I am pleased to provide you with free shipping for your o...

Read More

I would like to know if the viruses precipitated using the PEG Virus Precipitation Kit (ab102538) will be useable for sequencing purposes.

Thank you for contacting us. Yes, the concentrated virus produced by this kit can be used to produce purified viral DNA or RNA for sequencing. I hope this helps, please let me know if you need any additional information or assistance.

1 - Will the PEG remain in the concentraed Virus solution?
2 - Is choloform in any of the buffers?

Thank you for contacting Abcam.

I have talked to the lab regarding your enquiry and please find the responses below:

1 - Trace amounts of PEG might remain in the viral pellet from this kit. Trace amounts of PEG in the virus suspensi...

Read More

Thank you Thomas, I spoke with Qiagen as you suggested yesterday and ran my assay overnight as I was on a deadline. It worked fine. Thank you for your time.

That's good news! Let me know if you have any other questions.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up