Overview

  • Product name
    PEG Virus Precipitation Kit
  • Sample type
    Other biological fluids, Cell culture media
  • Assay type
    Direct
  • Sensitivity
    > 40 %
  • Range
    40 % - 100 %
  • Product overview

    Abcam's PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate viruses without ultra-centrifugation. The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer. The kit can be used to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples. Viruses can be concentrated over 100 fold. An optimized Virus Re-suspension Solution is provided to maximize viral recovery by 40-100% depending on the virus type and sources. The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Viruses are usually produced at a low titer. They often need to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate viruses and remove impurities.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 50 tests
    PEG Solution 1 x 125ml
    Virus Re-suspension Solution 1 x 10ml
  • Research areas
  • Relevance
    Virus is usually produced at a low titer. It often needs to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate virus and remove impurities. PEG (Polyethylene glycol) Virus Precipitation provides an easy, convenient and time-saving method to concentrate virus without ultra-centrifugation.
  • Alternative names
    • Polyethylene glycol virus

Images

  • Concentration of baculovirus: Low titer baculovirus (10 ml) expressing human Granzyme B was precipitated following the kit protocol and the precipitate was suspended in 1 ml of Virus Re-suspension Solution. Both low titer and precipitated baculovirus was subsequently used to infect SF9 Insect cells. The activity of recombinant Granzyme B secreted into the culture medium by low titer and precipitated baculovirus infected insect cells was monitored using ab157403 - Granzyme B Activity Assay Kit (Fluorometric).

Protocols

References

This product has been referenced in:
  • Rotem N  et al. ALS Along the Axons - Expression of Coding and Noncoding RNA Differs in Axons of ALS models. Sci Rep 7:44500 (2017). Read more (PubMed: 28300211) »
  • Gershoni-Emek N  et al. Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1. Mol Cell Proteomics 15:506-22 (2016). Functional Studies . Read more (PubMed: 26598648) »
See all 6 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

PEG Virus Kit works well

Excellent Excellent 5/5 (Ease of Use)
Abreviews
We used the Abcam PEG Virus Precipitation Kit (ab102538) to concentrate baculovirus. It worked well, no problems.

Dr. Ronald Gary

Verified customer

Submitted Apr 11 2019

PEG precipitation of Orthonairovirus

Good Excellent 5/5 (Ease of Use)
Abreviews
The protocol is simple and easy to follow, without the requirement for specialist equipment or buffers for precipiation.

Abcam user community

Verified customer

Submitted May 14 2018

Answer

You can use gentle sonication in a water bath to completely dissociate the pellet and then resuspend it in the virus resuspension solution. Make sure the sonication does not build heat in the sample.

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Answer

This PEG Virus Precipitation Kit (ab102538) can be used to concentrate viruses and is not specific to a particular type. Although we list retroviruses, baculoviruses, lentiviruses, and phages on our datasheet, the list does not stop there and this kit should also work on enteroviruses / Picornaviruses.

Please note that we do have a 1 year Abpromise guarantee on all our products and should you have any difficulties with it, please do not hesitate to contact us and we will be able to replace or refund the kit.

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Answer

The advantages of this PEG Virus Precipitation Kit are:
1. Ease of use, no need for ultracentrifugation and collection of the specific sucrose density layer.
2. High viral titer recovery and concentration.
3. Wide variety of viruses can be concentrated using this kit.
Please note we have not tested virus-like particles. It might be possible to concentrate them but this might need some optimization regarding volume of PEG solution and duration and speed of centrifugation used.

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Answer

We have not tested the PEG virus precipitation kit with samples post-flocculation but assuming the product of the flocculation is an aqueous solution, the kit should be compatible with this sample. However, it is unknown how much more concentration of the virus can be achieved with the PEG precipitation after a flocculation method, or the percentage recovery compared to using just the PEG without flocculation. The two methods are meant to accomplish the same thing.

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Question
Answer

If the presence of glycerol or salts is not going to work for your specific EM experiment that you can resuspend the virus pellet in your own buffer of choice.

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Answer

Thank you for your reply and my sincere apologies for my confusion.

I am sorry we are regrettably not able to provide samples for testing, as discussed in the previous email. However, I am pleased to provide you with free shipping for your order of ab102538 PEG Virus Precipitation Kit which I hope will be helpful. In order to obtain this discount, please use the following codes when you place the order:

Free shipping code: NO-PORTO-T7T61

I would like to reasure you that the kit will be covered by our 6 month guarantee. Also, you can obtain further reward by submitting an Abreview about this product via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers.

To find out more about our Abreview system and the Abpoints rewards you can obtain, please see the following webpage:

https://www.abcam.com/abreviews

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

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Answer

Thank you for contacting us. Yes, the concentrated virus produced by this kit can be used to produce purified viral DNA or RNA for sequencing. I hope this helps, please let me know if you need any additional information or assistance.

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Answer

Thank you for contacting Abcam.

I have talked to the lab regarding your enquiry and please find the responses below:

1 - Trace amounts of PEG might remain in the viral pellet from this kit. Trace amounts of PEG in the virus suspension will not affect the use of theconcentrated virus. In some cases, PEG may increase virus infection efficiency.However, if it is desired, the trace amount of PEG can be removed by the following procedure:

i) Add 1 volume of solution containing 4 M KCl and 50 mM Tris-HCl, pH7.2 (not

provided) to 3 volumes of the concentrated virus suspension.

ii) Alternatively, add solid KCl into the virus suspension to a final concentration of

1 M.

iii) Let stand on ice for 15 - 30 min.

iv) Spin at 12,000 x g for 10 min at 4°C to remove the precipitate.

v) Carefully collect the virus supernatant. Aliquot and store at -70°C for future use.



2 - There is no chloroform in any of the buffers in this kit.



Hope this information has been helpful for you.



Please let me know if you have any other questions

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1-10 of 12 Abreviews or Q&A

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