For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    peg-virus-precipitation-kit-ab102538.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits Molecular Biology Kits Virus Precipitation Kits
Share by email

PEG Virus Precipitation Kit (ab102538)

  • Datasheet
  • Protocol Booklet
Reviews (2)Q&A (8)References (12)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Functional Studies - PEG Virus Precipitation Kit (ab102538)

    Key features and details

    • Assay type: Direct
    • Sample type: Cell culture media, Other biological fluids
    • Sensitivity: 40 %

    You may also be interested in

    Conjugation
    Product image
    PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    ELISA
    Product image
    PEGylated Protein (Polyethylene Glycol) ELISA Kit (ab133065)
    Primary
    Product image
    Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (ab198933)

    View more associated products

    Overview

    • Product name

      PEG Virus Precipitation Kit
    • Sample type

      Other biological fluids, Cell culture media
    • Assay type

      Direct
    • Sensitivity

      > 40 %
    • Range

      40 % - 100 %
    • Product overview

      Abcam's PEG Virus Precipitation Kit provides an easy, convenient and time-saving method to concentrate viruses without ultra-centrifugation. The kit can be used for small lab samples or large scale virus preparation with high yield and high viral titer. The kit can be used to concentrate retroviruses, baculoviruses, lentiviruses, and phages etc. in cell culture medium or environmental samples. Viruses can be concentrated over 100 fold. An optimized Virus Re-suspension Solution is provided to maximize viral recovery by 40-100% depending on the virus type and sources. The whole process uses non-toxic reagents. The concentrated virus can be used for infection, viral DNA or RNA purification, etc.
      Visit our FAQs page for tips and troubleshooting.

    • Notes

      This product is manufactured by BioVision, an Abcam company and was previously called K904 PEG Virus Precipitation Kit. K904-50 is the same size as the 50 test size of ab102538.

      Viruses are usually produced at a low titer. They often need to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate viruses and remove impurities.

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 50 tests
      PEG Solution 1 x 125ml
      Virus Re-suspension Solution 1 x 10ml
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Molecular Biology Kits
      • Virus Precipitation Kits
    • Relevance

      Virus is usually produced at a low titer. It often needs to be concentrated for storage or further applications. A quick, easy and inexpensive method is desired to concentrate virus and remove impurities. PEG (Polyethylene glycol) Virus Precipitation provides an easy, convenient and time-saving method to concentrate virus without ultra-centrifugation.
    • Alternative names

      • Polyethylene glycol virus

    Images

    • Functional Studies - PEG Virus Precipitation Kit (ab102538)
      Functional Studies - PEG Virus Precipitation Kit (ab102538)

      Concentration of baculovirus: Low titer baculovirus (10 ml) expressing human Granzyme B was precipitated following the kit protocol and the precipitate was suspended in 1 ml of Virus Re-suspension Solution. Both low titer and precipitated baculovirus was subsequently used to infect SF9 Insect cells. The activity of recombinant Granzyme B secreted into the culture medium by low titer and precipitated baculovirus infected insect cells was monitored using ab157403 - Granzyme B Activity Assay Kit (Fluorometric).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (12)

    Publishing research using ab102538? Please let us know so that we can cite the reference in this datasheet.

    ab102538 has been referenced in 12 publications.

    • Zhang C  et al. KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia. Theranostics 11:2691-2705 (2021). PubMed: 33456567
    • Oguntuyo KY  et al. Quantifying Absolute Neutralization Titers against SARS-CoV-2 by a Standardized Virus Neutralization Assay Allows for Cross-Cohort Comparisons of COVID-19 Sera. mBio 12:N/A (2021). PubMed: 33593976
    • Ball DP  et al. Caspase-1 interdomain linker cleavage is required for pyroptosis. Life Sci Alliance 3:N/A (2020). PubMed: 32051255
    • Alvarez KLF  et al. An EdU-based flow cytometry assay to evaluate chicken T lymphocyte proliferation. BMC Vet Res 16:230 (2020). PubMed: 32631319
    • Oguntuyo KY  et al. Quantifying absolute neutralization titers against SARS-CoV-2 by a standardized virus neutralization assay allows for cross-cohort comparisons of COVID-19 sera. medRxiv N/A:N/A (2020). PubMed: 32817961
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 10 Abreviews or Q&A

    PEG Virus Kit works well

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    We used the Abcam PEG Virus Precipitation Kit (ab102538) to concentrate baculovirus. It worked well, no problems.

    DR. Ronald Gary

    Verified customer

    Submitted Apr 11 2019

    PEG precipitation of Orthonairovirus

    Good Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    The protocol is simple and easy to follow, without the requirement for specialist equipment or buffers for precipiation.

    Abcam user community

    Verified customer

    Submitted May 14 2018

    Question

    I am having trouble resuspending the pellet after centrifugation. Even when using a volume equal to the starting volume, the pellet does not want to go into solution. Do you have any suggestions?

    Read More

    Abcam community

    Verified customer

    Asked on May 22 2015

    Answer

    You can use gentle sonication in a water bath to completely dissociate the pellet and then resuspend it in the virus resuspension solution. Make sure the sonication does not build heat in the sample.

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on May 22 2015

    Question

    Have you tested the kit on enteroviruses / Picornaviruses?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 14 2015

    Answer

    This PEG Virus Precipitation Kit (ab102538) can be used to concentrate viruses and is not specific to a particular type. Although we list retroviruses, baculoviruses, lentiviruses, and phages on our datasheet, the list does not stop there and this kit should also work on enteroviruses / Picornaviruses.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Apr 14 2015

    Question

    1. Is there an advantage to using this product over sucrose cushion technique?
    2. Will this kit work to purify virus like particles?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 16 2014

    Answer

    The advantages of this PEG Virus Precipitation Kit are:
    1. Ease of use, no need for ultracentrifugation and collection of the specific sucrose density layer.
    2. High viral titer recovery and concentration.
    3. Wide variety of viruses can be concentrated using this kit.
    Please note we have not tested virus-like particles. It might be possible to concentrate them but this might need some optimization regarding volume of PEG solution and duration and speed of centrifugation used.

    Read More

    Jeremy Kasanov

    Abcam Scientific Support

    Answered on Apr 16 2014

    Question

    I would like to know if this kit can be used to concentrate virus after employing flocculation method.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 07 2014

    Answer

    We have not tested the PEG virus precipitation kit with samples post-flocculation but assuming the product of the flocculation is an aqueous solution, the kit should be compatible with this sample. However, it is unknown how much more concentration of the virus can be achieved with the PEG precipitation after a flocculation method, or the percentage recovery compared to using just the PEG without flocculation. The two methods are meant to accomplish the same thing.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Feb 07 2014

    Question

    Is the virus resuspension solution compatible with EM?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2013

    Answer

    If the presence of glycerol or salts is not going to work for your specific EM experiment that you can resuspend the virus pellet in your own buffer of choice.

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on Nov 14 2013

    Question

    I would like to know if the viruses precipitated using the PEG Virus Precipitation Kit (ab102538) will be useable for sequencing purposes.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 24 2012

    Answer

    Yes, the concentrated virus produced by this kit can be used to produce purified viral DNA or RNA for sequencing.

    Read More

    Abcam Scientific Support

    Answered on Sep 24 2012

    Question

    1 - Will the PEG remain in the concentraed Virus solution?
    2 - Is choloform in any of the buffers?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 21 2012

    Answer

    1 - Trace amounts of PEG might remain in the viral pellet from this kit. Trace amounts of PEG in the virus suspension will not affect the use of theconcentrated virus. In some cases, PEG may increase virus infection efficiency.However, if it is desired, the trace amount of PEG can be removed by the following procedure:

    i) Add 1 volume of solution containing 4 M KCl and 50 mM Tris-HCl, pH7.2 (not provided) to 3 volumes of the concentrated virus suspension.

    ii) Alternatively, add solid KCl into the virus suspension to a final concentration of 1 M.

    iii) Let stand on ice for 15 - 30 min.

    iv) Spin at 12,000 x g for 10 min at 4°C to remove the precipitate.

    v) Carefully collect the virus supernatant. Aliquot and store at -70°C for future use.


    2 - There is no chloroform in any of the buffers in this kit.

    Read More

    Abcam Scientific Support

    Answered on Aug 21 2012

    Question

    We would like to use this kit to isolate small, non-enveloped virus (DNA virus, circo virus, 20 to 30nm). Is this kit also suitable to precipitate this small viruses? Furthermore, do you have any information on the purity of the virus pellet?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 19 2011

    Answer

    1) We have not tested circovirus-but based on literature I think it should precipitate circovirus 2) Our kit formulation favors virus precipitation, but the purity (whether some proteins are co-precipitated) may depend on what is in the sample.

    Read More

    Abcam Scientific Support

    Answered on Aug 19 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.