PEG Virus Precipitation Kit (ab102538)

You can use gentle sonication in a water bath to completely dissociate the pellet and then resuspend it in the virus resuspension solution. Make sure the sonication does not build heat in the sample.

This PEG Virus Precipitation Kit (ab102538) can be used to concentrate viruses and is not specific to a particular type. Although we list retroviruses, baculoviruses, lentiviruses, and phages on our datasheet, the list does not stop there and this kit should also work on enteroviruses / Picornaviruses.

Please note that we do have a 1 year Abpromise guarantee on all our products and should you have any difficulties with it, please do not hesitate to contact us and we will be able to replace or refund the kit.

The advantages of this PEG Virus Precipitation Kit are:
1. Ease of use, no need for ultracentrifugation and collection of the specific sucrose density layer.
2. High viral titer recovery and concentration.
3. Wide variety of viruses can be concentrated using this kit.
Please note we have not tested virus-like particles. It might be possible to concentrate them but this might need some optimization regarding volume of PEG solution and duration and speed of centrifugation used.

We have not tested the PEG virus precipitation kit with samples post-flocculation but assuming the product of the flocculation is an aqueous solution, the kit should be compatible with this sample. However, it is unknown how much more concentration of the virus can be achieved with the PEG precipitation after a flocculation method, or the percentage recovery compared to using just the PEG without flocculation. The two methods are meant to accomplish the same thing.

If the presence of glycerol or salts is not going to work for your specific EM experiment that you can resuspend the virus pellet in your own buffer of choice.

Thank you for your reply and my sincere apologies for my confusion.

I am sorry we are regrettably not able to provide samples for testing, as discussed in the previous email. However, I am pleased to provide you with free shipping for your order of ab102538 PEG Virus Precipitation Kit which I hope will be helpful. In order to obtain this discount, please use the following codes when you place the order:

Free shipping code: NO-PORTO-T7T61

I would like to reasure you that the kit will be covered by our 6 month guarantee. Also, you can obtain further reward by submitting an Abreview about this product via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers.

To find out more about our Abreview system and the Abpoints rewards you can obtain, please see the following webpage:

https://www.abcam.com/abreviews

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

Thank you for contacting us. Yes, the concentrated virus produced by this kit can be used to produce purified viral DNA or RNA for sequencing. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for contacting Abcam.

I have talked to the lab regarding your enquiry and please find the responses below:

1 - Trace amounts of PEG might remain in the viral pellet from this kit. Trace amounts of PEG in the virus suspension will not affect the use of theconcentrated virus. In some cases, PEG may increase virus infection efficiency.However, if it is desired, the trace amount of PEG can be removed by the following procedure:

i) Add 1 volume of solution containing 4 M KCl and 50 mM Tris-HCl, pH7.2 (not

provided) to 3 volumes of the concentrated virus suspension.

ii) Alternatively, add solid KCl into the virus suspension to a final concentration of

1 M.

iii) Let stand on ice for 15 - 30 min.

iv) Spin at 12,000 x g for 10 min at 4°C to remove the precipitate.

v) Carefully collect the virus supernatant. Aliquot and store at -70°C for future use.



2 - There is no chloroform in any of the buffers in this kit.



Hope this information has been helpful for you.



Please let me know if you have any other questions