Overview

  • Product name

  • Description

    Rabbit polyclonal to PEG10/EDR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Giant Panda
  • Immunogen

    Synthetic peptide within Human PEG10/EDR aa 325-375. The exact sequence is proprietary.
    Database link: Q86TG7

  • Positive control

    • IP: HeLa whole cell lysate.
  • General notes

    ab226370 has not performed satisfactorily when used for WB of PEG10/EDR in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of PEG10/EDR.

     This product was previously labelled as PEG10

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.
    • Tissue specificity

      Expressed in the cytotrophoblast layer but not in the overlying syncytiotrophoblast of the placenta. Expressed in prostate and breast carcinomas but not in normal breast and prostate epithelial cells. Expressed in the HepG2 cell line (at protein level). Expressed in brain, liver, spleen, kidney, thymus, lung, ovary, testis, reactive lymph node, skeletal muscle, adipose tissue and placenta. Expressed in pancreatic and hepatocellular carcinomas (HCC).
    • Sequence similarities

      Contains 1 CCHC-type zinc finger.
    • Developmental stage

      Expressed in placenta during the first trimester of gestation (at protein level). In placenta, down-regulated at early hypoxic phase, and highly activated at 11-12 week of gestation.
    • Post-translational
      modifications

      Isoform RF1/RF2 undergoes proteolytic cleavage.
    • Cellular localization

      Nucleus. Cytoplasm. Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells and in the HepG2 cell line. Colocalized with ALK1.
    • Information by UniProt
    • Database links

    • Alternative names

      • AA407948 antibody
      • Edr antibody
      • Embryonal carcinoma differentiation regulated antibody
      • Embryonal carcinoma differentiation-regulated protein antibody
      • HB 1 antibody
      • HB1 antibody
      • KIAA1051 antibody
      • Mammalian retrotransposon-derived protein 2 antibody
      • Mar2 antibody
      • Mart2 antibody
      • MEF3 like 1 antibody
      • MEF3-like protein 1 antibody
      • MEF3L antibody
      • MEF3L1 antibody
      • MyEF 3 antibody
      • Myelin expression factor 3-like protein 1 antibody
      • Paternally expressed 10 antibody
      • Paternally expressed gene 10 ORF1 antibody
      • Paternally expressed gene 10 protein antibody
      • Peg10 antibody
      • PEG10 protein antibody
      • PEG10_HUMAN antibody
      • Putative uncharacterized protein PEG10 antibody
      • Retrotransposon gag domain containing 3 antibody
      • Retrotransposon gag domain-containing protein 3 antibody
      • Retrotransposon-derived gag-like polyprotein antibody
      • Retrotransposon-derived protein PEG10 antibody
      • RGAG3 antibody
      • Ty3/Gypsy-like protein antibody
      see all

    Images

    • PEG10/EDR was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226370 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226370 at 0.4 µg/ml.

      Lane 1: ab226370 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 3 minutes.

    References

    ab226370 has not yet been referenced specifically in any publications.

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