Overview

  • Product name

  • Description

    Mouse polyclonal to PEG10/EDR
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human PEG10/EDR.
    Database link: NP_001035242.1

  • Positive control

    • Human stomach tissue lysate, PEG10/EDR transfected 293T cell lysate.
  • General notes

     This product was previously labelled as PEG10

     

Properties

Applications

Our Abpromise guarantee covers the use of ab68780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 42, 100 kDa (predicted molecular weight: 37 kDa).

Target

  • Function

    Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.
  • Tissue specificity

    Expressed in the cytotrophoblast layer but not in the overlying syncytiotrophoblast of the placenta. Expressed in prostate and breast carcinomas but not in normal breast and prostate epithelial cells. Expressed in the HepG2 cell line (at protein level). Expressed in brain, liver, spleen, kidney, thymus, lung, ovary, testis, reactive lymph node, skeletal muscle, adipose tissue and placenta. Expressed in pancreatic and hepatocellular carcinomas (HCC).
  • Sequence similarities

    Contains 1 CCHC-type zinc finger.
  • Developmental stage

    Expressed in placenta during the first trimester of gestation (at protein level). In placenta, down-regulated at early hypoxic phase, and highly activated at 11-12 week of gestation.
  • Post-translational
    modifications

    Isoform RF1/RF2 undergoes proteolytic cleavage.
  • Cellular localization

    Nucleus. Cytoplasm. Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells and in the HepG2 cell line. Colocalized with ALK1.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA407948 antibody
    • Edr antibody
    • Embryonal carcinoma differentiation regulated antibody
    • Embryonal carcinoma differentiation-regulated protein antibody
    • HB 1 antibody
    • HB1 antibody
    • KIAA1051 antibody
    • Mammalian retrotransposon-derived protein 2 antibody
    • Mar2 antibody
    • Mart2 antibody
    • MEF3 like 1 antibody
    • MEF3-like protein 1 antibody
    • MEF3L antibody
    • MEF3L1 antibody
    • MyEF 3 antibody
    • Myelin expression factor 3-like protein 1 antibody
    • Paternally expressed 10 antibody
    • Paternally expressed gene 10 ORF1 antibody
    • Paternally expressed gene 10 protein antibody
    • Peg10 antibody
    • PEG10 protein antibody
    • PEG10_HUMAN antibody
    • Putative uncharacterized protein PEG10 antibody
    • Retrotransposon gag domain containing 3 antibody
    • Retrotransposon gag domain-containing protein 3 antibody
    • Retrotransposon-derived gag-like polyprotein antibody
    • Retrotransposon-derived protein PEG10 antibody
    • RGAG3 antibody
    • Ty3/Gypsy-like protein antibody
    see all

Images

  • Anti-PEG10/EDR antibody (ab68780) at 1/500 dilution + Human stomach tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate
    at 1/2500 dilution

    Predicted band size: 37 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-PEG10/EDR antibody (ab68780) at 1/500 dilution

    Lane 1 : PEG10/EDR transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?

References

ab68780 has not yet been referenced specifically in any publications.

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