Key features and details
- Mouse polyclonal to PEG10/EDR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PEG10/EDR antibody
See all PEG10/EDR primary antibodies
DescriptionMouse polyclonal to PEG10/EDR
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human PEG10/EDR.
Database link: NP_001035242.1
- Human stomach tissue lysate, PEG10/EDR transfected 293T cell lysate.
This product was previously labelled as PEG10
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 42, 100 kDa (predicted molecular weight: 37 kDa).|
FunctionPrevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.
Tissue specificityExpressed in the cytotrophoblast layer but not in the overlying syncytiotrophoblast of the placenta. Expressed in prostate and breast carcinomas but not in normal breast and prostate epithelial cells. Expressed in the HepG2 cell line (at protein level). Expressed in brain, liver, spleen, kidney, thymus, lung, ovary, testis, reactive lymph node, skeletal muscle, adipose tissue and placenta. Expressed in pancreatic and hepatocellular carcinomas (HCC).
Sequence similaritiesContains 1 CCHC-type zinc finger.
Developmental stageExpressed in placenta during the first trimester of gestation (at protein level). In placenta, down-regulated at early hypoxic phase, and highly activated at 11-12 week of gestation.
modificationsIsoform RF1/RF2 undergoes proteolytic cleavage.
Cellular localizationNucleus. Cytoplasm. Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells and in the HepG2 cell line. Colocalized with ALK1.
- Information by UniProt
- AA407948 antibody
- Edr antibody
- Embryonal carcinoma differentiation regulated antibody
Anti-PEG10/EDR antibody (ab68780) at 1/500 dilution + Human stomach tissue lysate at 50 µg
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
at 1/2500 dilution
Predicted band size: 37 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
All lanes : Anti-PEG10/EDR antibody (ab68780) at 1/500 dilution
Lane 1 : PEG10/EDR transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 37 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
ab68780 has not yet been referenced specifically in any publications.