Recombinant Anti-PEG10/EDR antibody [EPR20051] (ab215035)
![Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-389433-anti-peg10edr-antibody-epr20051-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20051] to PEG10/EDR
- Suitable for: ICC/IF, IP, Flow Cyt, IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PEG10/EDR antibody [EPR20051]
See all PEG10/EDR primary antibodies -
Description
Rabbit monoclonal [EPR20051] to PEG10/EDR -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human PEG10/EDR aa 400-700. The exact sequence is proprietary.
Database link: Q86TG7 -
Positive control
- WB: HEK 293, HeLa and HepG2 whole cell lysates; Human testis lysate. IHC-P: Human placenta, liver cancer and testis tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells. IP: HepG2 whole cell lysate.
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General notes
This product was previously labelled as PEG10
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20051 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab215035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500. | |
| IP | 1/30. | |
| Flow Cyt | 1/500. | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 30,80 kDa). |
Target
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Function
Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter. -
Tissue specificity
Expressed in the cytotrophoblast layer but not in the overlying syncytiotrophoblast of the placenta. Expressed in prostate and breast carcinomas but not in normal breast and prostate epithelial cells. Expressed in the HepG2 cell line (at protein level). Expressed in brain, liver, spleen, kidney, thymus, lung, ovary, testis, reactive lymph node, skeletal muscle, adipose tissue and placenta. Expressed in pancreatic and hepatocellular carcinomas (HCC). -
Sequence similarities
Contains 1 CCHC-type zinc finger. -
Developmental stage
Expressed in placenta during the first trimester of gestation (at protein level). In placenta, down-regulated at early hypoxic phase, and highly activated at 11-12 week of gestation. -
Post-translational
modificationsIsoform RF1/RF2 undergoes proteolytic cleavage. -
Cellular localization
Nucleus. Cytoplasm. Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells and in the HepG2 cell line. Colocalized with ALK1. - Information by UniProt
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Database links
- Entrez Gene: 23089 Human
- Omim: 609810 Human
- SwissProt: Q86TG7 Human
- Unigene: 147492 Human
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Alternative names
- AA407948 antibody
- Edr antibody
- Embryonal carcinoma differentiation regulated antibody
see all
Images
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Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)All lanes : Anti-PEG10/EDR antibody [EPR20051] (ab215035) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PEG10 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 30,80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab215035 observed at 100 kDa. Red - loading control ab7291 observed at 50 kDa.
ab215035 Anti-PEG10/EDR antibody [EPR20051] was shown to specifically react with PEG10/EDR in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265925 (knockout cell lysate ab258103) was used. Wild-type and PEG10/EDR knockout samples were subjected to SDS-PAGE. ab215035 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-268748-anti-peg10-antibody-epr20051-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on part of the cells in human liver cancer is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-268745-if-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PEG10/EDR antibody [EPR20051] (ab215035)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-268751-anti-peg10-antibody-epr20051-western-blot.jpg)
Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)Lanes 1-2 : Anti-PEG10/EDR antibody [EPR20051] (ab215035) at 1/2000 dilution
Lane 3 : Anti-PEG10/EDR antibody [EPR20051] (ab215035) at 1/10000 dilution
Lane 1 : HEK 293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30,80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression of PEG10/EDR in HepG2 cells is consistent with what has been described in the literature PMID: 17942406.
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![Flow Cytometry - Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-268744-anti-peg10-antibody-epr20051-flow-cytometry.jpg)
Flow Cytometry - Anti-PEG10/EDR antibody [EPR20051] (ab215035)Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling PEG10/EDR with ab215035 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-268750-anti-peg10-antibody-epr20051-western-blot.jpg)
Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)Anti-PEG10/EDR antibody [EPR20051] (ab215035) at 1/1000 dilution + Human testis lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 30,80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-268749-anti-peg10-antibody-epr20051-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on some cells in human placenta is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-268747-anti-peg10-antibody-epr20051-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on the germ cells in human testis is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-268746-anti-peg10-antibody-epr20051-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Negative staining on normal cells in human liver.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-268743-anti-peg10-antibody-epr20051-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PEG10/EDR antibody [EPR20051] (ab215035)PEG10/EDR was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab215035 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215035 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: HepG2 whole cell lysate, 10 μg (Input).
Lane 2: ab215035 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215035 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Anti-PEG10/EDR antibody [EPR20051] (ab215035)](https://www.abcam.com/ps/products/215/ab215035/Images/ab215035-11-benefits-of-recombinant-antibodies.png)
Anti-PEG10/EDR antibody [EPR20051] (ab215035)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab215035 has not yet been referenced specifically in any publications.
