Recombinant
RabMAb

Recombinant Anti-PEG10/EDR antibody [EPR20051] (ab215035)

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Overview

  • Product name

    Anti-PEG10/EDR antibody [EPR20051]
    See all PEG10/EDR primary antibodies

  • Description

    Rabbit monoclonal [EPR20051] to PEG10/EDR

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human PEG10/EDR aa 400-700. The exact sequence is proprietary.
    Database link: Q86TG7

  • Positive control

    • WB: HEK 293, HeLa and HepG2 whole cell lysates; Human testis lysate. IHC-P: Human placenta, liver cancer and testis tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells. IP: HepG2 whole cell lysate.

  • General notes

     This product was previously labelled as PEG10

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
IP 1/30.
Flow Cyt 1/500.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 30,80 kDa).

Target

  • Function

    Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.

  • Tissue specificity

    Expressed in the cytotrophoblast layer but not in the overlying syncytiotrophoblast of the placenta. Expressed in prostate and breast carcinomas but not in normal breast and prostate epithelial cells. Expressed in the HepG2 cell line (at protein level). Expressed in brain, liver, spleen, kidney, thymus, lung, ovary, testis, reactive lymph node, skeletal muscle, adipose tissue and placenta. Expressed in pancreatic and hepatocellular carcinomas (HCC).

  • Sequence similarities

    Contains 1 CCHC-type zinc finger.

  • Developmental stage

    Expressed in placenta during the first trimester of gestation (at protein level). In placenta, down-regulated at early hypoxic phase, and highly activated at 11-12 week of gestation.

  • Post-translational
    modifications

    Isoform RF1/RF2 undergoes proteolytic cleavage.

  • Cellular localization

    Nucleus. Cytoplasm. Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells and in the HepG2 cell line. Colocalized with ALK1.
  • Information by UniProt

  • Database links

    • Alternative names

      • AA407948 antibody
      • Edr antibody
      • Embryonal carcinoma differentiation regulated antibody
      • Embryonal carcinoma differentiation-regulated protein antibody
      • HB 1 antibody
      • HB1 antibody
      • KIAA1051 antibody
      • Mammalian retrotransposon-derived protein 2 antibody
      • Mar2 antibody
      • Mart2 antibody
      • MEF3 like 1 antibody
      • MEF3-like protein 1 antibody
      • MEF3L antibody
      • MEF3L1 antibody
      • MyEF 3 antibody
      • Myelin expression factor 3-like protein 1 antibody
      • Paternally expressed 10 antibody
      • Paternally expressed gene 10 ORF1 antibody
      • Paternally expressed gene 10 protein antibody
      • Peg10 antibody
      • PEG10 protein antibody
      • PEG10_HUMAN antibody
      • Putative uncharacterized protein PEG10 antibody
      • Retrotransposon gag domain containing 3 antibody
      • Retrotransposon gag domain-containing protein 3 antibody
      • Retrotransposon-derived gag-like polyprotein antibody
      • Retrotransposon-derived protein PEG10 antibody
      • RGAG3 antibody
      • Ty3/Gypsy-like protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)

      Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on part of the cells in human liver cancer is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Immunocytochemistry/ Immunofluorescence - Anti-PEG10/EDR antibody [EPR20051] (ab215035)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Lanes 1-2 : Anti-PEG10/EDR antibody [EPR20051] (ab215035) at 1/2000 dilution
      Lane 3 : Anti-PEG10/EDR antibody [EPR20051] (ab215035) at 1/10000 dilution

      Lane 1 : HEK 293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 30,80 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The expression of PEG10/EDR in HepG2 cells is consistent with what has been described in the literature PMID: 17942406.

    • Flow Cytometry - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Flow Cytometry - Anti-PEG10/EDR antibody [EPR20051] (ab215035)

      Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling PEG10/EDR with ab215035 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    • Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Western blot - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Anti-PEG10/EDR antibody [EPR20051] (ab215035) at 1/1000 dilution + Human testis lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

      Predicted band size: 30,80 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)

      Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on some cells in human placenta is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on the germ cells in human testis is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEG10/EDR antibody [EPR20051] (ab215035)

      Immunohistochemical analysis of paraffin-embedded human liver tissue labeling PEG10/EDR with ab215035 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

      Negative staining on normal cells in human liver.

      Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-PEG10/EDR antibody [EPR20051] (ab215035)
      Immunoprecipitation - Anti-PEG10/EDR antibody [EPR20051] (ab215035)

      PEG10/EDR was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab215035 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215035 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

      Lane 1: HepG2 whole cell lysate, 10 μg (Input).

      Lane 2: ab215035 IP in HepG2 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215035 in HepG2 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

    References

    ab215035 has not yet been referenced specifically in any publications.

    Publishing research using ab215035? Please let us know so that we can cite the reference in this datasheet.

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