PEGylated Protein (Polyethylene Glycol) ELISA Kit (ab133065)
Key features and details
- Range: 1.75 ng/ml - 225 ng/ml
- Sample type: Other biological fluids, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
PEGylated Protein (Polyethylene Glycol) ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 25ng/ml 3.4% 2 16 50ng/ml 4.2% Inter-assay Sample n Mean SD CV% Expect conc1 3 113ng/ml 0.4 2.4% Expect conc1 3 27.7ng/ml 1.4 1.3% Expect conc1 3 7.25ng/ml 3.1 5.7% -
Sample type
Serum, Plasma, Other biological fluids, Tissue -
Assay type
Competitive -
Range
1.75 ng/ml - 225 ng/ml -
Assay time
2h 00m -
Assay duration
Multiple steps standard assay -
Product overview
PEGylated protein ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of PEGylated protein in plasma, serum, tissue lysate samples and other biological samples.
The kit detects both free and conjugated forms of PEG. Due to the nature of the antibody used, this assay is more sensitive to higher molecular weight PEG molecules.
A monoclonal antibody specific to PEGylated protein has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of PEGylated protein conjugated to biotin, followed by a solution of polyclonal antibody to PEGylated protein. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a blue color. A stop solution changes this color from blue to yellow, and the intensity of this yellow coloration is inversely proportional to the amount of PEGylated protein captured in the plate.
This kit does not contain Tween 20.
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents when performing ELISA using anti-PEG products.
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Notes
Poly(ethylene glycol) (PEG) is a widely used polymer in drug delivery systems and is often directly conjugated to a drug therapeutic. PEGylation of drug therapeutics is desirable as it has been found to increase the retention time, reduce the immunogenicity and increase stability towards metabolic enzymes. ab133065, the PEGylated protein ELISA Kit, is applicable for drug development and pharmaceutical manufacturing applications including drug formulations, pharmacokinetics analysis, drug comparison, lead candidate identification, lot release criteria and in-process QC studies. At this time, there are no long-term studies revealing the fate of this polymer in the body. The need for toxicology studies that would determine the accumulation of the metabolized PEGylated therapeutics in various tissues has been identified.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Assay Buffer 39 1 x 25ml 20X Wash Buffer 3 Concentrate 2 x 25ml 500X PEGylated Protein Conjugate 1 x 50µl Biotinylated PEGylated Protein 1 x 5ml PEG Microtiter Plate 1 unit PEGylated BSA Control 1 x 50µl PEGylated Protein Conjugate Diluent 1 x 12ml Plate Sealer 2 units Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Alternative names
- Polyethylene Glycol
Images
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Typical Standard Curve
Representative standard curve using ab133065.
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Typical Standard CurveRepresentative standard curve using ab133065.
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Typical Standard CurveRepresentative standard curve using ab133065.
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Typical Standard CurveRepresentative standard curve using ab133065.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab133065 has been referenced in 2 publications.
- Golder MR et al. Reduction of liver fibrosis by rationally designed macromolecular telmisartan prodrugs. Nat Biomed Eng 2:822-830 (2018). PubMed: 30918745
- Wufuer Y et al. GPVI-Fc-PEG improves cerebral infarct volume and cerebral thrombosis in mouse model with cerebral thrombosis. Mol Med Rep 16:7561-7568 (2017). PubMed: 28944903