Product nameAnti-Pellino 1 antibody
See all Pellino 1 primary antibodies
DescriptionRabbit polyclonal to Pellino 1
Tested applicationsSuitable for: ICC, ICC/IF, Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis
- Human Ramos cell lysate, mouse Raw cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody is affinity purified.
Our Abpromise guarantee covers the use of ab13812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/1000. Predicted molecular weight: 46 kDa.|
FunctionScaffold protein involved in the IL-1 signaling pathway via its interaction with the complex containing IRAK kinases and TRAF6. Required for NF-kappa-B activation and IL-8 gene expression in response to IL-1.
Sequence similaritiesBelongs to the pellino family.
- Information by UniProt
- E3 ubiquitin-protein ligase pellino homolog 1 antibody
- PELI1 antibody
- PELI1_HUMAN antibody
ab13812 staining Pellino 1 in RAW264.7 cells from Mouse macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal(1/1000) was used as the secondary antibody..
All lanes : Anti-Pellino 1 antibody (ab13812) at 1/1000 dilution
Lane 1 : Lysates prepared from human Ramos cell line
Lane 2 : Lysates prepared from mouse Raw cell line
Predicted band size: 46 kDa
Western blot analysis of Pellino1 in human Ramos cell lysate (A) and mouse Raw cell lysate (B) using ab13812 at 1/1000
This product has been referenced in:
- Ma X et al. Interaction of the oncogenic miR-21 microRNA and the p53 tumor suppressor pathway. Carcinogenesis 34:1216-23 (2013). Mouse . Read more (PubMed: 23385064) »
- Kinsella S et al. Bid Promotes K63-Linked Polyubiquitination of Tumor Necrosis Factor Receptor Associated Factor 6 (TRAF6) and Sensitizes to Mutant SOD1-Induced Proinflammatory Signaling in Microglia. eNeuro 3:N/A (0). Read more (PubMed: 27257617) »