Flow Cytometry abreview for Anti-Pellino 1 antibody

Good
Abreviews
Application
Flow Cytometry
Sample
Human Cell (Platelets)
Specification
Platelets
Preparation
Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Platelets

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Flour488 Chicken Anti Rabbit IgG (H+L)
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Abbreviations in Fig
P : Permeabilized
US : Unstained (Red Peak)
IGG RB : IgG Rabbit (Blue Peak)
PELLINO Ab (Green Peak)

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Submitted Dec 22 2010

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