Western blot abreview for Anti-Pellino 1 antibody

Average
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS/0.05% Tween

Secondary antibody

Name
Non-Abcam antibody was used: Pierce 31210
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Bands
Specific: 46 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
HEK293 cells were transfected or not with FLAG-Pellino1

Additional data

Additional Notes
This antibody recognises over expressed Pellino1. It is difficult to detect endogenous Pellino1 as its expressed at low levels . Over-exposing this WB did not show a band in untransfected cells.

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Submitted Aug 19 2013

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