Anti-Pellino 1 antibody - C-terminal (ab189953)
Key features and details
- Rabbit polyclonal to Pellino 1 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pellino 1 antibody - C-terminal
See all Pellino 1 primary antibodies -
Description
Rabbit polyclonal to Pellino 1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Pellino 1 (C terminal). (15 amino acid peptide near the carboxy terminus; NP_065702.2).
Database link: Q96FA3 -
Positive control
- Human tonsil tissue; HepG2 cells; Human liver tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC |
Use a concentration of 2.5 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
ICC
Use a concentration of 2.5 µg/ml. |
Target
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Function
Scaffold protein involved in the IL-1 signaling pathway via its interaction with the complex containing IRAK kinases and TRAF6. Required for NF-kappa-B activation and IL-8 gene expression in response to IL-1. -
Sequence similarities
Belongs to the pellino family. - Information by UniProt
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Database links
- Entrez Gene: 57162 Human
- Omim: 614797 Human
- SwissProt: Q96FA3 Human
- Unigene: 727530 Human
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Alternative names
- E3 ubiquitin-protein ligase pellino homolog 1 antibody
- PELI1 antibody
- PELI1_HUMAN antibody
see all
Images
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Lane 1 : Anti-Pellino 1 antibody - C-terminal (ab189953) at 1 µg/ml
Lane 2 : Anti-Pellino 1 antibody - C-terminal (ab189953) at 2 µg/ml
All lanes : Human liver tissue lysate
Predicted band size: 46 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling Pellino 1 with ab189953 at 5 µg/ml.
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Immunofluorescent analysis of HepG2 cells labeling Pellino 1 with ab189953 at 20 µg/ml.
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Immunocytochemical analysis of HepG2 cells labeling Pellino 1 with ab189953 at 2.5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab189953 has not yet been referenced specifically in any publications.