Overview

  • Product name

  • Description

    Rabbit polyclonal to Pellino 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 166-395 of Human Pellino 2 (Q9HAT8).

  • Positive control

    • HeLa, HepG2 and HCT116 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab126982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.

Target

  • Relevance

    Scaffold protein which probably links Toll like receptors (TLRs) to basic cellular processes via its interaction with the complex containing IRAK (IL1R associated kinase) kinases and TRAF6. Can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1. Not required for NF-kappa-B activation.
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase pellino homolog 2 antibody
    • PELI2 antibody
    • pellino E3 ubiquitin protein ligase family member 2 antibody
    • Pellino homolog 2 antibody

Images

  • Anti-Pellino 2 antibody (ab126982) at 1/500 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE

References

ab126982 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 19 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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