Overview

  • Product name

  • Description

    Rabbit polyclonal to PELO
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-276 of Human PELO (UniProt: Q9BRX2).

  • Positive control

    • A431 and Jurkat whole cell lysates; A431 cells; Human HBL435 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Required for normal chromosome segregation during cell division and genomic stability (By similarity). May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. May have ribonuclease activity.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the eukaryotic release factor 1 family. Pelota subfamily.
  • Domain

    The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CGI 17 antibody
    • CGI17 antibody
    • pelo antibody
    • PELO_HUMAN antibody
    • pelota (Drosophila) homolog antibody
    • pelota homolog antibody
    • PRO1770 antibody
    • Protein pelota homolog antibody
    see all

Images

  • All lanes : Anti-PELO antibody (ab154335) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Jurkat whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 43 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells (4% at RT; 15mins), labeling PELO with ab154335 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human HBL435 xenograft tissue, labeling PELO with ab154335 at 1/500 dilution.

References

ab154335 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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