Anti-PELO antibody (ab241167)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PELO antibody
See all PELO primary antibodies -
Description
Rabbit polyclonal to PELO -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human PELO aa 1-50. The exact sequence is proprietary.
Database link: Q9BRX2 -
Positive control
- IP: Jurkat cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab241167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Required for normal chromosome segregation during cell division and genomic stability (By similarity). May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. May have ribonuclease activity. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the eukaryotic release factor 1 family. Pelota subfamily. -
Domain
The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 53918 Human
- Omim: 605757 Human
- SwissProt: Q9BRX2 Human
- SwissProt: Q5RCE3 Orangutan
- Unigene: 644352 Human
- Unigene: 669791 Human
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Alternative names
- CGI 17 antibody
- CGI17 antibody
- pelo antibody
see all
Images
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Immunoprecipitation - Anti-PELO antibody (ab241167)PELO was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241167 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241167 at 1 µg/ml.
Lane 1: ab241167 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Datasheets and documents
References
ab241167 has not yet been referenced specifically in any publications.
