Anti-PELO antibody (ab241167)
Key features and details
- Rabbit polyclonal to PELO
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PELO antibody
See all PELO primary antibodies -
Description
Rabbit polyclonal to PELO -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human PELO aa 1-50. The exact sequence is proprietary.
Database link: Q9BRX2 -
Positive control
- IP: Jurkat cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab241167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Required for normal chromosome segregation during cell division and genomic stability (By similarity). May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. May have ribonuclease activity. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the eukaryotic release factor 1 family. Pelota subfamily. -
Domain
The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 53918 Human
- Omim: 605757 Human
- SwissProt: Q9BRX2 Human
- SwissProt: Q5RCE3 Orangutan
- Unigene: 644352 Human
- Unigene: 669791 Human
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Alternative names
- CGI 17 antibody
- CGI17 antibody
- pelo antibody
see all
Images
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Immunoprecipitation - Anti-PELO antibody (ab241167)
PELO was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241167 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241167 at 1 µg/ml.
Lane 1: ab241167 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241167 has not yet been referenced specifically in any publications.
