Overview

  • Product name

    Anti-PELO antibody [EP7530]
    See all PELO primary antibodies
  • Description

    Rabbit monoclonal [EP7530] to PELO
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to N terminal amino acids of Human PELO (UniProt: Q9BRX2).

  • Positive control

    • HeLa, 293T, A375 and Human fetal lung lysates; Human liver and squamous cell cervical carcinoma tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP7530
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Required for normal chromosome segregation during cell division and genomic stability (By similarity). May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. May have ribonuclease activity.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the eukaryotic release factor 1 family. Pelota subfamily.
    • Domain

      The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity.
    • Cellular localization

      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • CGI 17 antibody
      • CGI17 antibody
      • pelo antibody
      • PELO_HUMAN antibody
      • pelota (Drosophila) homolog antibody
      • pelota homolog antibody
      • PRO1770 antibody
      • Protein pelota homolog antibody
      see all

    Images

    • All lanes : Anti-PELO antibody [EP7530] (ab140615) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Human fetal lung lysate
      Lane 4 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 43 kDa

    • Immunohistochemical analysis of paraffin embedded Human liver tissue labelling PELO with ab140615 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin embedded Human squamous cell cervical carcinoma tissue labelling PELO with ab140615 at 1/250 dilution.

    References

    This product has been referenced in:

    • O'Connell AE  et al. Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation. PLoS Genet 15:e1007917 (2019). Read more (PubMed: 30707697) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab140615.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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