Overview

  • Product name

    Pembrolizumab ELISA Kit  (Keytruda®)
    See all Pembrolizumab kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 15%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 15%
  • Sample type

    Serum, Plasma
  • Assay type

    Quantitative
  • Sensitivity

    10 ng/ml
  • Range

    30 ng/ml - 1000 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85% - 115%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Pembrolizumab ELISA Kit (Keytruda®) (ab237652) is a highly specific and sensitive kit designed for the in vitro determination of Pembrolizumab in human serum and plasma. The density of color is proportional to the amount of Pembrolizumab captured from the samples and can be quantified when compared with standard curve.


    Pembrolizumab (Keytruda®) is an antibody drug that targets the cell surface receptor programmed cell death protein 1 (PD-1) found on T cells. By preventing the binding of its ligands (PD-L1 and PD-L2), Pembrolizumab induces an anti-tumor immune response. Upregulation of PD-1 ligands is a mechanism for tumors to evade anti-tumor immune response; when PD-1 binds its ligand, the T cell receives an inhibitory signal leading to T cell anergy and blockade of anti-tumour immune response. Instead of directly targeting tumor tissue to induce tumor cell death, Pembrolizumab acts as a checkpoint inhibitor to stimulate immune responses to eliminate cancer cells.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 96 tests
    Assay Buffer 1 x 50ml
    HRP-conjugate Probe 1 x 12ml
    Micro ELISA Plate 1 unit
    Pembrolizumab Standard S1 1 x 0.3ml
    Pembrolizumab Standard S2 1 x 0.3ml
    Pembrolizumab Standard S3 1 x 0.3ml
    Pembrolizumab Standard S4 1 x 0.3ml
    Pembrolizumab Standard S5 1 x 0.3ml
    Pembrolizumab Standard S6 1 x 0.3ml
    Pembrolizumab Standard S7 1 x 0.3ml
    Plate sealers 2 units
    Stop Solution 1 x 12ml
    TMB substrate  1 x 12ml
    Wash buffer (20X) 1 x 50ml
  • Relevance

    Pembrolizumab (Keytruda®) is an antibody drug that targets the cell surface receptor programmed cell death protein 1 (PD-1) found on T cells. By preventing the binding of its ligands (PD-L1 and PD-L2), pembrolizumab induces an antitumor immune response. Upregulation of PD-1 ligands is a mechanism for tumors to evade antitumor immune response; when PD-1 binds its ligand, the T cell receives an inhibitory signal leading to T cell anergy and blockade of anti-tumour immune response. Instead of directly targeting tumor tissue to induce tumor cell death, pembrolizumab acts as a checkpoint inhibitor to stimulate immune responses to eliminate cancer cells.
  • Alternative names

    • Keytruda

Applications

Our Abpromise guarantee covers the use of ab237652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.

Protocols

References

ab237652 has not yet been referenced specifically in any publications.

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