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    pen2-antibody-ab18189.pdf

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Anti-PEN2 antibody (ab18189)

  • Datasheet
Reviews (5) Submit a question References (11)

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Western blot - Anti-PEN2 antibody (ab18189)
  • Immunocytochemistry/ Immunofluorescence - Anti-PEN2 antibody (ab18189)

Key features and details

  • Rabbit polyclonal to PEN2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-PEN2 antibody [EPR9200] (ab154830)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-PEN2 antibody
    See all PEN2 primary antibodies
  • Description

    Rabbit polyclonal to PEN2
  • Host species

    Rabbit
  • Specificity

    ab18189 specifically detects mouse 12kDa Pen2 protein in lung lysate. A number of higher MW bands are also detected (~50 and ~100kDa bands) which may be because a proportion of Pen2 is found bound to components of the gamma secretase complex (see the Relevance section). All bands detected with ab18189 in mouse lung lysate were blocked with the immunising peptide ab19147. Human Pen2 protein was also detected (weakly) in lung lysates with ab18189 (2ug/ml) in our hands and all detected bands blocked by immunising peptide ab19147.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • For endogenous sample testing in (e.g. in lung, spleen or brain tissue), it is suggested that the investigator load a greater amount of protein on the gel and combine immunoprecipitation with Western blot analysis. This antibody gave a positive result in HeLa cell line in ICC/IF
  • General notes


    PEN2 is a functional component of the gamma-secretase complex; coimmunoprecipitation of endogenous PEN2 and PS1 fragments using PS1 antibodies has been reported (Steiner et al, 2002).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    PEN2 is a functional component of the gamma-secretase complex; coimmunoprecipitation of endogenous PEN2 and PS1 fragments using PS1 antibodies has been reported (Steiner et al, 2002).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Neuroscience
    • Diseases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PEN2 protein (ab132019)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
Use a concentration of 2 µg/ml. Detects a band of approximately 11.9 kDa (predicted molecular weight: 12 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 11.9 kDa (predicted molecular weight: 12 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity.
  • Tissue specificity

    Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
  • Sequence similarities

    Belongs to the PEN-2 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
  • Target information above from: UniProt accession Q9NZ42 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55851 Human
    • Entrez Gene: 66340 Mouse
    • Entrez Gene: 292788 Rat
    • Entrez Gene: 81751 Rat
    • Omim: 607632 Human
    • SwissProt: Q9NZ42 Human
    • SwissProt: Q9CQR7 Mouse
    • SwissProt: O88777 Rat
    • SwissProt: Q6QI68 Rat
    • Unigene: 534465 Human
    • Unigene: 86557 Mouse
    • Unigene: 1240 Rat
    see all
  • Alternative names

    • Gamma secretase subunit PEN 2 antibody
    • Gamma Secretase Subunit PEN2 antibody
    • Gamma-secretase subunit PEN-2 antibody
    • Hematopoietic stem/progenitor cells protein MDS033 antibody
    • MDS033 antibody
    • MSTP064 antibody
    • PEN 2 antibody
    • PEN2_HUMAN antibody
    • Presenilin Enhancer 2 antibody
    • Presenilin enhancer 2 homolog antibody
    • Presenilin enhancer protein 2 antibody
    • PSEN2 antibody
    • psenen antibody
    see all

Images

  • Western blot - Anti-PEN2 antibody (ab18189)
    Western blot - Anti-PEN2 antibody (ab18189)
    All lanes : Anti-PEN2 antibody (ab18189) at 2 µg/ml

    Lane 1 : Mouse Lung Lysate
    Lane 2 : Mouse Lung Lysate with Human PEN2 peptide (ab19147) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor Goat polyclonal to Rabbit at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 12 kDa
    Observed band size: 12 kDa
    Additional bands at: 50-60 kDa, 95-100 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-PEN2 antibody (ab18189)
    Immunocytochemistry/ Immunofluorescence - Anti-PEN2 antibody (ab18189)
    ICC/IF image of ab18189 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18189, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (11)

Publishing research using ab18189? Please let us know so that we can cite the reference in this datasheet.

ab18189 has been referenced in 11 publications.

  • Sun Y  et al. Beclin-1 improves mitochondria-associated membranes in the heart during endotoxemia. FASEB Bioadv 3:123-135 (2021). PubMed: 33733054
  • Escamilla-Ayala AA  et al. Super-resolution microscopy reveals majorly mono- and dimeric presenilin1/?-secretase at the cell surface. Elife 9:N/A (2020). PubMed: 32631487
  • Hur JY  et al. The innate immunity protein IFITM3 modulates ?-secretase in Alzheimer's disease. Nature 586:735-740 (2020). PubMed: 32879487
  • Bannai T  et al. Chronic cerebral hypoperfusion shifts the equilibrium of amyloid ß oligomers to aggregation-prone species with higher molecular weight. Sci Rep 9:2827 (2019). PubMed: 30808940
  • Wong E  et al. GSAP modulates ?-secretase specificity by inducing conformational change in PS1. Proc Natl Acad Sci U S A 116:6385-6390 (2019). PubMed: 30850537
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-PEN2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Loading amount
20 µg
Specification
MEF
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More

Abcam user community

Verified customer

Submitted Nov 25 2016

Western blot abreview for Anti-PEN2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (Brain membrane preparations)
Loading amount
20 µg
Specification
Brain membrane preparations
Gel Running Conditions
Reduced Denaturing (12% Tris-glycine)
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 22°C
Read More

Mr. Michael Ho

Verified customer

Submitted Jul 10 2009

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