Anti-PEN2 antibody - Carboxyterminal end (ab62514)
Key features and details
- Rabbit polyclonal to PEN2 - Carboxyterminal end
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PEN2 antibody - Carboxyterminal end
See all PEN2 primary antibodies -
Description
Rabbit polyclonal to PEN2 - Carboxyterminal end -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (Human) of 13 amino acids from near the C-terminus of PEN2
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Positive control
- K562 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
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ICC/IF |
Use a concentration of 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). |
ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity. -
Tissue specificity
Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. -
Sequence similarities
Belongs to the PEN-2 family. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi. - Information by UniProt
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Database links
- Entrez Gene: 55851 Human
- Omim: 607632 Human
- SwissProt: Q9NZ42 Human
- Unigene: 534465 Human
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Alternative names
- Gamma secretase subunit PEN 2 antibody
- Gamma Secretase Subunit PEN2 antibody
- Gamma-secretase subunit PEN-2 antibody
see all
Images
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Lane 1 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 0.5 µg/ml
Lane 2 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 1 µg/ml
Lane 3 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 2 µg/ml
All lanes : K562 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 12 kDa
Observed band size: 12 kDa -
Immunofluorescence of K562 cells using ab62514 at 20 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab62514 has been referenced in 2 publications.
- Elad N et al. The dynamic conformational landscape of gamma-secretase. J Cell Sci 128:589-98 (2015). PubMed: 25501811
- Area-Gomez E et al. Presenilins are enriched in endoplasmic reticulum membranes associated with mitochondria. Am J Pathol 175:1810-6 (2009). WB ; Mouse . PubMed: 19834068