Recombinant Anti-PEN2 antibody [EPR9200] (ab154830)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9200] to PEN2
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PEN2 antibody [EPR9200]
See all PEN2 primary antibodies -
Description
Rabbit monoclonal [EPR9200] to PEN2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Neuro-2a, fetal lung and Human placenta lysates; Human skeletal muscle tissue; Neuro-2a cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9200 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
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IHC-P | (3) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 12 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity. -
Tissue specificity
Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. -
Sequence similarities
Belongs to the PEN-2 family. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi. - Information by UniProt
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Database links
- Entrez Gene: 55851 Human
- Entrez Gene: 66340 Mouse
- Entrez Gene: 292788 Rat
- Entrez Gene: 81751 Rat
- Omim: 607632 Human
- SwissProt: Q9NZ42 Human
- SwissProt: Q9CQR7 Mouse
- SwissProt: O88777 Rat
see all -
Alternative names
- Gamma secretase subunit PEN 2 antibody
- Gamma Secretase Subunit PEN2 antibody
- Gamma-secretase subunit PEN-2 antibody
see all
Images
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All lanes : Anti-PEN2 antibody [EPR9200] (ab154830) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : PSENEN knockout HEK-293 cell lysate
Lane 3 : Neuro-2a cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDaFalse colour image of Western blot: Anti-PEN2 antibody [EPR9200] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab154830 was shown to bind specifically to PEN2. A band was observed at 12 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in PSENEN knockout cell line ab273720 (knockout cell lysate ab273772). To generate this image, wild-type and PSENEN knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-PEN2 antibody [EPR9200] (ab154830) at 1/1000 dilution
Lane 1 : Neuro-2a cell lysate
Lane 2 : Fetal lung tissue lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 12 kDa -
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PEN2 with ab154830 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized Neuro-2a cells labeling PEN2 with ab154830 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab154830 has been referenced in 5 publications.
- Ma T et al. Low-dose metformin targets the lysosomal AMPK pathway through PEN2. Nature 603:159-165 (2022). PubMed: 35197629
- Xu TH et al. Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to ?-secretase cleavage and the Aß42/Aß40 ratio. Cell Discov 2:16026 (2016). WB ; Human . PubMed: 27625790
- Zhang X et al. The topology of pen-2, a ?-secretase subunit, revisited: evidence for a reentrant loop and a single pass transmembrane domain. Mol Neurodegener 10:39 (2015). PubMed: 26296997
- Holmes O et al. Functional analysis and purification of a Pen-2 fusion protein for ?-secretase structural studies. J Neurochem 131:94-100 (2014). PubMed: 24865334
- Holmes O et al. Pen-2 is essential for ?-secretase complex stability and trafficking but partially dispensable for endoproteolysis. Biochemistry 53:4393-406 (2014). PubMed: 24941111