Recombinant Anti-PEN2 antibody [EPR9200] (ab154830)
![Western blot - Anti-PEN2 antibody [EPR9200] (ab154830)](https://www.abcam.com/ps/products/154/ab154830/Images/ab154830-161409-anti-pen2-antibody-epr9200-western-blot.jpg "Western blot - Anti-PEN2 antibody [EPR9200] (ab154830)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9200] to PEN2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-PEN2 antibody [EPR9200]
See all PEN2 primary antibodies -
Description
Rabbit monoclonal [EPR9200] to PEN2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide corresponding to residues in Human PEN2 (UniProt Q9NZ42).
-
Positive control
- Neuro-2a, fetal lung and Human placenta lysates; Human skeletal muscle tissue; Neuro-2a cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9200 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
|
|
| IHC-P | (3) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 12 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Application notes
Is unsuitable for IP.
Target
-
Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity. -
Tissue specificity
Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. -
Sequence similarities
Belongs to the PEN-2 family. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi. - Information by UniProt
-
Database links
- Entrez Gene: 55851 Human
- Entrez Gene: 66340 Mouse
- Entrez Gene: 292788 Rat
- Entrez Gene: 81751 Rat
- Omim: 607632 Human
- SwissProt: Q9NZ42 Human
- SwissProt: Q9CQR7 Mouse
- SwissProt: O88777 Rat
see all -
Alternative names
- Gamma secretase subunit PEN 2 antibody
- Gamma Secretase Subunit PEN2 antibody
- Gamma-secretase subunit PEN-2 antibody
see all
Images
-
Western blot - Anti-PEN2 antibody [EPR9200] (ab154830)All lanes : Anti-PEN2 antibody [EPR9200] (ab154830) at 1/1000 dilution
Lane 1 : Neuro-2a cell lysate
Lane 2 : Fetal lung lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 12 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEN2 antibody [EPR9200] (ab154830)](https://www.abcam.com/ps/products/154/ab154830/Images/ab154830-161408-anti-pen2-antibody-epr9200-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEN2 antibody [EPR9200] (ab154830)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PEN2 with ab154830 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Flow Cytometry - Anti-PEN2 antibody [EPR9200] (ab154830)](https://www.abcam.com/ps/products/154/ab154830/Images/ab154830-161407-anti-pen2-antibody-epr9200-flow-cytometry.jpg)
Flow Cytometry - Anti-PEN2 antibody [EPR9200] (ab154830)Flow cytometric analysis of permeabilized Neuro-2a cells labeling PEN2 with ab154830 at 1/100 dilution (red) compared to a rabbit IgG negative control (green). -
![Anti-PEN2 antibody [EPR9200] (ab154830)](https://www.abcam.com/ps/products/154/ab154830/Images/ab154830-4-benefits-of-recombinant-antibodies.png)
Anti-PEN2 antibody [EPR9200] (ab154830)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab154830 has been referenced in 4 publications.
- Xu TH et al. Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to ?-secretase cleavage and the Aß42/Aß40 ratio. Cell Discov 2:16026 (2016). WB ; Human . PubMed: 27625790
- Zhang X et al. The topology of pen-2, a ?-secretase subunit, revisited: evidence for a reentrant loop and a single pass transmembrane domain. Mol Neurodegener 10:39 (2015). PubMed: 26296997
- Holmes O et al. Functional analysis and purification of a Pen-2 fusion protein for ?-secretase structural studies. J Neurochem 131:94-100 (2014). PubMed: 24865334
- Holmes O et al. Pen-2 is essential for ?-secretase complex stability and trafficking but partially dispensable for endoproteolysis. Biochemistry 53:4393-406 (2014). PubMed: 24941111