Recombinant Anti-Pentraxin 3/PTX3 antibody [EPR6699] (ab125007)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6699] to Pentraxin 3/PTX3
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Pentraxin 3/PTX3 antibody [EPR6699]
See all Pentraxin 3/PTX3 primary antibodies -
Description
Rabbit monoclonal [EPR6699] to Pentraxin 3/PTX3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Pentraxin 3/PTX3 aa 50-150. The exact sequence is proprietary.
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Positive control
- HL-60 treated with Dexamethasone, HL-60, Human spleen, NIH/3T3, and HUVEC lysates; HUVEC cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6699 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
Target
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Function
Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. -
Sequence similarities
Contains 1 pentaxin domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5806 Human
- Entrez Gene: 19288 Mouse
- Omim: 602492 Human
- SwissProt: P26022 Human
- SwissProt: P48759 Mouse
- Unigene: 591286 Human
- Unigene: 276776 Mouse
- Unigene: 442151 Mouse
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Alternative names
- AI607804 antibody
- Pentaxin 3 antibody
- Pentaxin-related protein PTX3 antibody
see all
Images
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Anti-Pentraxin 3/PTX3 antibody [EPR6699] (ab125007) at 1/10000 dilution + NIH/3T3 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 42 kDa -
Anti-Pentraxin 3/PTX3 antibody [EPR6699] (ab125007) at 1/10000 dilution + HUVEC cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 42 kDa -
All lanes : Anti-Pentraxin 3/PTX3 antibody [EPR6699] (ab125007) at 1/1000 dilution (unpurified)
Lane 1 : HL-60 treated with Dexamethasone lysate
Lane 2 : HL-60 lysate
Lane 3 : Human spleen lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : HUVEC lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab125007 has been referenced in 8 publications.
- Estébanez B et al. Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects. Antioxidants (Basel) 9:N/A (2020). PubMed: 32012711
- Wu XY et al. Complement C1q synergizes with PTX3 in promoting NLRP3 inflammasome over-activation and pyroptosis in rheumatoid arthritis. J Autoimmun 106:102336 (2020). PubMed: 31601476
- Lu Y et al. Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain. J Neurosci 40:7355-7374 (2020). PubMed: 32817249
- Johnson TK et al. Exosomes derived from induced vascular progenitor cells promote angiogenesis in vitro and in an in vivo rat hindlimb ischemia model. Am J Physiol Heart Circ Physiol 317:H765-H776 (2019). PubMed: 31418583
- Bendícková K et al. Calcineurin inhibitors reduce NFAT-dependent expression of antifungal pentraxin-3 by human monocytes. J Leukoc Biol N/A:N/A (2019). PubMed: 30934147
- Song T et al. Pentraxin 3 overexpression accelerated tumor metastasis and indicated poor prognosis in hepatocellular carcinoma via driving epithelial-mesenchymal transition. J Cancer 9:2650-2658 (2018). PubMed: 30087705
- Tian F et al. Constitutive activated STAT3 is an essential regulator and therapeutic target in esophageal squamous cell carcinoma. Oncotarget 8:88719-88729 (2017). WB . PubMed: 29179470
- Zhou Y et al. Calcium dobesilate may alleviate diabetes-induced endothelial dysfunction and inflammation. Mol Med Rep 16:8635-8642 (2017). PubMed: 29039485