Recombinant
RabMAb

Anti-Pentraxin 3 antibody [EPR6699] (ab125007)

Overview

  • Product name
    Anti-Pentraxin 3 antibody [EPR6699]
    See all Pentraxin 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR6699] to Pentraxin 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Pentraxin 3 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • HL-60 treated with Dexamethasone, HL-60, Human spleen, NIH/3T3, and HUVEC lysates; HUVEC cells
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab125007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
    • Sequence similarities
      Contains 1 pentaxin domain.
    • Post-translational
      modifications
      Glycosylated.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI607804 antibody
      • Pentaxin 3 antibody
      • Pentaxin-related protein PTX3 antibody
      • Pentraxin 3 long antibody
      • Pentraxin-related gene antibody
      • Pentraxin-related gene rapidly induced by IL-1 beta antibody
      • Pentraxin-related protein PTX3 antibody
      • PTX3 antibody
      • PTX3_HUMAN antibody
      • TNF alpha-induced protein 5 antibody
      • TNFAIP5 antibody
      • TSG-14 antibody
      • TSG14 antibody
      • Tumor necrosis factor alpha-induced protein 5 antibody
      • Tumor necrosis factor-inducible gene 14 protein antibody
      • Tumor necrosis factor-inducible protein TSG-14 antibody
      see all

    Images

    • Anti-Pentraxin 3 antibody [EPR6699] (ab125007) at 1/10000 dilution + NIH/3T3 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 42 kDa

    • Anti-Pentraxin 3 antibody [EPR6699] (ab125007) at 1/10000 dilution + HUVEC cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 42 kDa

    • All lanes : Anti-Pentraxin 3 antibody [EPR6699] (ab125007) at 1/1000 dilution (unpurified)

      Lane 1 : HL-60 treated with Dexamethasone lysate
      Lane 2 : HL-60 lysate
      Lane 3 : Human spleen lysate
      Lane 4 : NIH/3T3 cell lysate
      Lane 5 : HUVEC lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Tian F  et al. Constitutive activated STAT3 is an essential regulator and therapeutic target in esophageal squamous cell carcinoma. Oncotarget 8:88719-88729 (2017). WB . Read more (PubMed: 29179470) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (cardiac tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    30 µg
    Treatment
    LPS treated mice
    Specification
    cardiac tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted May 07 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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