Recombinant
RabMAb

Recombinant Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

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Overview

  • Product name

    Anti-Pentraxin 3/PTX3 antibody [EPR18678-105]
    See all Pentraxin 3/PTX3 primary antibodies

  • Description

    Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Pentraxin 3/PTX3 aa 1-200. The exact sequence is proprietary.
    Database link: P26022
    (Peptide available as ab85335)

  • Positive control

    • WB: HUVEC whole cell lysate (+/- treatment with LPS and BFA). ICC/IF: HUVEC cells. Flow cytometry: HUVEC cells. IP: HUVEC whole cell lysate.

  • General notes

    Previously labelled as Pentraxin 3. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/60.
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).Can be blocked with Recombinant Human Pentraxin 3/PTX3 protein (ab85335).
IP 1/30.

Target

  • Function

    Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.

  • Sequence similarities

    Contains 1 pentaxin domain.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • AI607804 antibody
      • Pentaxin 3 antibody
      • Pentaxin-related protein PTX3 antibody
      • Pentraxin 3 long antibody
      • Pentraxin-related gene antibody
      • Pentraxin-related gene rapidly induced by IL-1 beta antibody
      • Pentraxin-related protein PTX3 antibody
      • PTX3 antibody
      • PTX3_HUMAN antibody
      • TNF alpha-induced protein 5 antibody
      • TNFAIP5 antibody
      • TSG-14 antibody
      • TSG14 antibody
      • Tumor necrosis factor alpha-induced protein 5 antibody
      • Tumor necrosis factor-inducible gene 14 protein antibody
      • Tumor necrosis factor-inducible protein TSG-14 antibody
      see all

    Images

    • Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      All lanes : Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838) at 1/1000 dilution

      Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
      Lane 2 : HUVEC treated with 0.5 ug/ml LPS for 4 hours followed by 300 ng/ml BFA for 20 hours, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 41 kDa
      Observed band size: 42 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking and dilution buffer: 5% NFDM/TBST 

    • Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HUVEC cell line.

      The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (red).


      -ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

       

    • Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838) at 1/200 dilution + HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 41 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking and dilution buffer: 5% NFDM/TBST

      Higher concentration of this antibody is required for the detection of Pentraxin 3/PTX3 in untreated cells in WB.

    • Flow Cytometry - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Flow Cytometry - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    • Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

      Pentraxin 3/PTX3 was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) whole cell lysate with ab190838 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190838 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.


      Lane 1: HUVEC whole cell lysate 10 μg (Input).
      Lane 2: ab190838 IP in HUVEC whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190838 in HUVEC whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 minutes.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 17389238).

    References

    ab190838 has not yet been referenced specifically in any publications.

    Publishing research using ab190838? Please let us know so that we can cite the reference in this datasheet.

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