Recombinant Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3
- Suitable for: ICC/IF, Flow Cyt, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Pentraxin 3/PTX3 antibody [EPR18678-105]
See all Pentraxin 3/PTX3 primary antibodies -
Description
Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- WB: HUVEC whole cell lysate (+/- treatment with LPS and BFA). ICC/IF: HUVEC cells. Flow cytometry: HUVEC cells. IP: HUVEC whole cell lysate.
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General notes
Previously labelled as Pentraxin 3.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18678-105 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab190838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| Flow Cyt | 1/60. | |
| WB | 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).Can be blocked with Recombinant Human Pentraxin 3/PTX3 protein (ab85335). | |
| IP | 1/30. |
Target
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Function
Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. -
Sequence similarities
Contains 1 pentaxin domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5806 Human
- Omim: 602492 Human
- SwissProt: P26022 Human
- Unigene: 591286 Human
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Alternative names
- AI607804 antibody
- Pentaxin 3 antibody
- Pentaxin-related protein PTX3 antibody
see all
Images
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![Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-288609-190838-new.jpg)
Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)All lanes : Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838) at 1/1000 dilution
Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : HUVEC treated with 0.5 ug/ml LPS for 4 hours followed by 300 ng/ml BFA for 20 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and dilution buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-288279-190838b.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HUVEC cell line.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (red).
-ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
![Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-288278-190838a.jpg)
Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838) at 1/200 dilution + HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and dilution buffer: 5% NFDM/TBST
Higher concentration of this antibody is required for the detection of Pentraxin 3/PTX3 in untreated cells in WB.
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![Flow Cytometry - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-288280-190838c.jpg)
Flow Cytometry - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-288281-190838d.jpg)
Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)Pentraxin 3/PTX3 was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) whole cell lysate with ab190838 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190838 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HUVEC whole cell lysate 10 μg (Input).
Lane 2: ab190838 IP in HUVEC whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190838 in HUVEC whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.The molecular weight observed is consistent with what has been described in the literature (PMID: 17389238).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References
ab190838 has not yet been referenced specifically in any publications.
