RecombinantRabMAb

Recombinant Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

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Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
  • Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
  • Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
  • Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
  • Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3
  • Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Pentraxin 3/PTX3 antibody [EPR18678-105]
    See all Pentraxin 3/PTX3 primary antibodies

  • Description

    Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab85335)

  • Positive control

    • WB: HUVEC whole cell lysate (+/- treatment with LPS and BFA). ICC/IF: HUVEC cells. Flow Cyt (intra): HUVEC cells. IP: HUVEC whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).Can be blocked with Recombinant Human Pentraxin 3/PTX3 protein (ab85335).
IP
1/30.
Flow Cyt (Intra)
1/60.
Notes
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).Can be blocked with Recombinant Human Pentraxin 3/PTX3 protein (ab85335).
IP
1/30.
Flow Cyt (Intra)
1/60.

Target

  • Function

    Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.

  • Sequence similarities

    Contains 1 pentaxin domain.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • AI607804 antibody
      • Pentaxin 3 antibody
      • Pentaxin-related protein PTX3 antibody
      • Pentraxin 3 long antibody
      • Pentraxin-related gene antibody
      • Pentraxin-related gene rapidly induced by IL-1 beta antibody
      • Pentraxin-related protein PTX3 antibody
      • PTX3 antibody
      • PTX3_HUMAN antibody
      • TNF alpha-induced protein 5 antibody
      • TNFAIP5 antibody
      • TSG-14 antibody
      • TSG14 antibody
      • Tumor necrosis factor alpha-induced protein 5 antibody
      • Tumor necrosis factor-inducible gene 14 protein antibody
      • Tumor necrosis factor-inducible protein TSG-14 antibody
      see all

    Images

    • Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      All lanes : Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838) at 1/1000 dilution

      Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
      Lane 2 : HUVEC treated with 0.5 ug/ml LPS for 4 hours followed by 300 ng/ml BFA for 20 hours, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 41 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking and dilution buffer: 5% NFDM/TBST 

    • Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HUVEC cell line.

      The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (red).


      -ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

       

    • Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838) at 1/200 dilution + HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 41 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking and dilution buffer: 5% NFDM/TBST

      Higher concentration of this antibody is required for the detection of Pentraxin 3/PTX3 in untreated cells in WB.

    • Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

      Pentraxin 3/PTX3 was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) whole cell lysate with ab190838 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190838 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.


      Lane 1: HUVEC whole cell lysate 10 μg (Input).
      Lane 2: ab190838 IP in HUVEC whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190838 in HUVEC whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 minutes.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 17389238).

    • Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
      Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab190838? Please let us know so that we can cite the reference in this datasheet.

    ab190838 has been referenced in 2 publications.

    • Herskind C  et al. Gene Expression Profiles Reveal Extracellular Matrix and Inflammatory Signaling in Radiation-Induced Premature Differentiation of Human Fibroblast in vitro. Front Cell Dev Biol 9:539893 (2021). PubMed: 33681189
    • Chui A  et al. Oxidative stress regulates progenitor behavior and cortical neurogenesis. Development 147:N/A (2020). PubMed: 32041791

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