Recombinant Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
![Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-288609-190838-new.jpg "Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3
- Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Pentraxin 3/PTX3 antibody [EPR18678-105]
See all Pentraxin 3/PTX3 primary antibodies -
Description
Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab85335) -
Positive control
- WB: HUVEC whole cell lysate (+/- treatment with LPS and BFA). ICC/IF: HUVEC cells. Flow Cyt (intra): HUVEC cells. IP: HUVEC whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18678-105 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
|
|
| WB |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).Can be blocked with Recombinant Human Pentraxin 3/PTX3 protein (ab85335).
|
|
| IP |
1/30.
|
|
| Flow Cyt (Intra) |
1/60.
|
| Notes |
|---|
|
ICC/IF
1/100. |
|
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).Can be blocked with Recombinant Human Pentraxin 3/PTX3 protein (ab85335). |
|
IP
1/30. |
|
Flow Cyt (Intra)
1/60. |
Target
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Function
Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. -
Sequence similarities
Contains 1 pentaxin domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5806 Human
- Omim: 602492 Human
- SwissProt: P26022 Human
- Unigene: 591286 Human
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Alternative names
- AI607804 antibody
- Pentaxin 3 antibody
- Pentaxin-related protein PTX3 antibody
see all
Images
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Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)All lanes : Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838) at 1/1000 dilution
Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : HUVEC treated with 0.5 ug/ml LPS for 4 hours followed by 300 ng/ml BFA for 20 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and dilution buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-288279-190838b.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HUVEC cell line.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (red).
-ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
![Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-288278-190838a.jpg)
Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838) at 1/200 dilution + HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and dilution buffer: 5% NFDM/TBST
Higher concentration of this antibody is required for the detection of Pentraxin 3/PTX3 in untreated cells in WB.
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![Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-288280-190838c.jpg)
Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-288281-190838d.jpg)
Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)Pentraxin 3/PTX3 was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) whole cell lysate with ab190838 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190838 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HUVEC whole cell lysate 10 μg (Input).
Lane 2: ab190838 IP in HUVEC whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190838 in HUVEC whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.The molecular weight observed is consistent with what has been described in the literature (PMID: 17389238).
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![Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)](https://www.abcam.com/ps/products/190/ab190838/Images/ab190838-6-benefits-of-recombinant-antibodies.png)
Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] (ab190838)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab190838 has been referenced in 2 publications.
- Herskind C et al. Gene Expression Profiles Reveal Extracellular Matrix and Inflammatory Signaling in Radiation-Induced Premature Differentiation of Human Fibroblast in vitro. Front Cell Dev Biol 9:539893 (2021). PubMed: 33681189
- Chui A et al. Oxidative stress regulates progenitor behavior and cortical neurogenesis. Development 147:N/A (2020). PubMed: 32041791
