Recombinant
RabMAb

Recombinant Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)

Overview

  • Product name

    Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free
    See all Pentraxin 3/PTX3 primary antibodies
  • Description

    Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity


    Predicted to work with: Human
  • Immunogen

    Recombinant fragment within Human Pentraxin 3/PTX3 aa 1-200. The exact sequence is proprietary.
    Database link: P26022

  • Positive control

    • ICC/IF: HUVEC cells.
  • General notes

    Ab226866 is the carrier-free version of ab190838. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).

Target

  • Function

    Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
  • Sequence similarities

    Contains 1 pentaxin domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI607804 antibody
    • Pentaxin 3 antibody
    • Pentaxin-related protein PTX3 antibody
    • Pentraxin 3 long antibody
    • Pentraxin-related gene antibody
    • Pentraxin-related gene rapidly induced by IL-1 beta antibody
    • Pentraxin-related protein PTX3 antibody
    • PTX3 antibody
    • PTX3_HUMAN antibody
    • TNF alpha-induced protein 5 antibody
    • TNFAIP5 antibody
    • TSG-14 antibody
    • TSG14 antibody
    • Tumor necrosis factor alpha-induced protein 5 antibody
    • Tumor necrosis factor-inducible gene 14 protein antibody
    • Tumor necrosis factor-inducible protein TSG-14 antibody
    see all

Images

  • Pentraxin 3/PTX3 was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) whole cell lysate with ab190838 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190838 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.


    Lane 1: HUVEC whole cell lysate 10 μg (Input).
    Lane 2: ab190838 IP in HUVEC whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190838 in HUVEC whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 17389238).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HUVEC cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (red).


    -ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).

References

ab226866 has not yet been referenced specifically in any publications.

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