Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
Overview
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Product name
Anti-Pentraxin 3/PTX3 antibody [MNB1]
See all Pentraxin 3/PTX3 primary antibodies -
Description
Rat monoclonal [MNB1] to Pentraxin 3/PTX3 -
Host species
Rat -
Tested applications
Suitable for: ICC/IF, WB, IP, IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Pentraxin 3/PTX3.
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Positive control
- ICC/IF: MDA-MB-231 cells.
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General notes
Previously labelled as Pentraxin 3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered antibody solution. -
Clonality
Monoclonal -
Clone number
MNB1 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab90806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/50. Predicted molecular weight: 42 kDa. | |
| IP | Use at an assay dependent concentration. | |
| IHC-Fr | 1/50. | |
| IHC-P | 1/50. |
Target
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Function
Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. -
Sequence similarities
Contains 1 pentaxin domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5806 Human
- Omim: 602492 Human
- SwissProt: P26022 Human
- Unigene: 591286 Human
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Alternative names
- AI607804 antibody
- Pentaxin 3 antibody
- Pentaxin-related protein PTX3 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)](https://www.abcam.com/ps/products/90/ab90806/Images/ab90806-212961-anti-pentraxin-3-ptx3-antibody-mnb1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)ICC/IF image of ab90806 stained MDA-MB-231 cells. The cells were 4% parafrmaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90806, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab98420, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References
ab90806 has been referenced in 5 publications.
- Bendícková K et al. Calcineurin inhibitors reduce NFAT-dependent expression of antifungal pentraxin-3 by human monocytes. J Leukoc Biol N/A:N/A (2019). PubMed: 30934147
- Scimeca M et al. Impairment of PTX3 expression in osteoblasts: a key element for osteoporosis. Cell Death Dis 8:e3125 (2017). WB, IHC, ICC/IF ; Human . PubMed: 29022895
- Mayer C et al. Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells. Sci Rep 6:37128 (2016). WB ; Human . PubMed: 27849015
- Belone Ade F et al. Genome-Wide Screening of mRNA Expression in Leprosy Patients. Front Genet 6:334 (2015). IHC ; Human . PubMed: 26635870
- Foo SS et al. Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation. PLoS Pathog 11:e1004649 (2015). PubMed: 25695775
