Recombinant Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
![Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)](https://www.abcam.com/ps/products/90/ab90806/Images/ab90806-449374-anti-pentraxin-3-ptx3-antibody-mnb1-western-blot-huvec-lps-brefeldin-a-treated-human.jpg "Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [MNB1] to Pentraxin 3/PTX3
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Pentraxin 3/PTX3 antibody [MNB1]
See all Pentraxin 3/PTX3 primary antibodies -
Description
Rat monoclonal [MNB1] to Pentraxin 3/PTX3 -
Host species
Rat -
Tested applications
Suitable for: WB, IHC-Frmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Pentraxin 3/PTX3.
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Positive control
- WB: HUVEC, 3T3-L1 and MDA-MB-231 whole cell lysate. LPS, Brefeldin treated HUVEC whole cell lysate. Mouse and rat placenta tissue lysate. IHC-Fr: Mouse and rat placenta tissue.
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General notes
This product has switched from a hybridoma to recombinant production method on 10th December 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MNB1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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| IHC-Fr |
1/20.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
|
IHC-Fr
1/20. |
Target
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Function
Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. -
Sequence similarities
Contains 1 pentaxin domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5806 Human
- Entrez Gene: 19288 Mouse
- Entrez Gene: 689388 Rat
- Omim: 602492 Human
- SwissProt: P26022 Human
- SwissProt: P48759 Mouse
- Unigene: 591286 Human
- Unigene: 276776 Mouse
see all -
Alternative names
- AI607804 antibody
- Pentaxin 3 antibody
- Pentaxin-related protein PTX3 antibody
see all
Images
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Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)All lanes : Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806) at 1/1000 dilution
Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : HUVEC treated with 0.5ug/ml Lipopolysaccharides(LPS) for 4 hours and then added 300 ng/ml Brefeldin A for another 20 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 41 kDa
Exposure time: 15 secondsThe expression profile observed is consistent with what has been described in the literature (PMID:17389238).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)](https://www.abcam.com/ps/products/90/ab90806/Images/ab90806-449373-anti-pentraxin-3-ptx3-antibody-mnb1-immunohistochemistry-frozen-placenta-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse placenta tissue labeling Pentraxin 3/PTX3 with ab90806 at 1/20 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on mouse placenta was observed. Nuclear counterstain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. -
![Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)](https://www.abcam.com/ps/products/90/ab90806/Images/ab90806-449372-anti-pentraxin-3-ptx3-antibody-mnb1-western-blot-mda-mb-231-human-3t3-l1-placenta-mouse-rat.jpg)
Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)All lanes : Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806) at 1/1000 dilution
Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Mouse placenta tissue lysate
Lane 3 : Rat placenta tissue lysate
Lane 4 : 3T3-L1 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1, 2: 15 seconds; Lane 3: 3 seconds; Lane 4: 180 seconds.
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![Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)](https://www.abcam.com/ps/products/90/ab90806/Images/ab90806-449371-anti-pentraxin-3-ptx3-antibody-mnb1-immunohistochemistry-frozen-placenta-rat.jpg)
Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen rat placenta tissue labeling Pentraxin 3/PTX3 with ab90806 at 1/20 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on rat placenta was observed. Nuclear counterstain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Protocols
Datasheets and documents
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Datasheet download
References (10)
ab90806 has been referenced in 10 publications.
- Miyazaki Y et al. Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model. Sci Rep 11:4690 (2021). PubMed: 33633222
- Naito T et al. Mesenchymal stem cells induce tumor stroma formation and epithelial-mesenchymal transition through SPARC expression in colorectal cancer. Oncol Rep 45:N/A (2021). PubMed: 33907853
- Chang X et al. Pentraxin 3 is a diagnostic and prognostic marker for ovarian epithelial cancer patients based on comprehensive bioinformatics and experiments. Cancer Cell Int 21:193 (2021). PubMed: 33952272
- Kendal AR et al. Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon. Sci Rep 10:13939 (2020). PubMed: 32883960
- Wu XY et al. Complement C1q synergizes with PTX3 in promoting NLRP3 inflammasome over-activation and pyroptosis in rheumatoid arthritis. J Autoimmun 106:102336 (2020). PubMed: 31601476
- Bendícková K et al. Calcineurin inhibitors reduce NFAT-dependent expression of antifungal pentraxin-3 by human monocytes. J Leukoc Biol N/A:N/A (2019). PubMed: 30934147
- Scimeca M et al. Impairment of PTX3 expression in osteoblasts: a key element for osteoporosis. Cell Death Dis 8:e3125 (2017). WB, IHC, ICC/IF ; Human . PubMed: 29022895
- Mayer C et al. Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells. Sci Rep 6:37128 (2016). WB ; Human . PubMed: 27849015
- Belone Ade F et al. Genome-Wide Screening of mRNA Expression in Leprosy Patients. Front Genet 6:334 (2015). IHC ; Human . PubMed: 26635870
- Foo SS et al. Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation. PLoS Pathog 11:e1004649 (2015). PubMed: 25695775
