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    pentraxin-3ptx3-antibody-mnb1-ab90806.pdf

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Recombinant

Recombinant Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)

  • Datasheet
  • SDS
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Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
  • Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
  • Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
  • Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [MNB1] to Pentraxin 3/PTX3
  • Suitable for: WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Pentraxin 3/PTX3 antibody [MNB1]
    See all Pentraxin 3/PTX3 primary antibodies
  • Description

    Rat monoclonal [MNB1] to Pentraxin 3/PTX3
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, IHC-Frmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Pentraxin 3/PTX3.

  • Positive control

    • WB: HUVEC, 3T3-L1 and MDA-MB-231 whole cell lysate. LPS, Brefeldin treated HUVEC whole cell lysate. Mouse and rat placenta tissue lysate. IHC-Fr: Mouse and rat placenta tissue.
  • General notes

    This product has switched from a hybridoma to recombinant production method on 10th December 2020.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    MNB1
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Innate and adaptive immunity
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Pentraxin 3/PTX3 antibody [MNB1] - BSA and Azide free (ab264487)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab90806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-Fr
1/20.
Notes
WB
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-Fr
1/20.
Application notes
Is unsuitable for ICC.

Target

  • Function

    Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
  • Sequence similarities

    Contains 1 pentaxin domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P26022 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5806 Human
    • Entrez Gene: 19288 Mouse
    • Entrez Gene: 689388 Rat
    • Omim: 602492 Human
    • SwissProt: P26022 Human
    • SwissProt: P48759 Mouse
    • Unigene: 591286 Human
    • Unigene: 276776 Mouse
    • Unigene: 442151 Mouse
    see all
  • Alternative names

    • AI607804 antibody
    • Pentaxin 3 antibody
    • Pentaxin-related protein PTX3 antibody
    • Pentraxin 3 long antibody
    • Pentraxin-related gene antibody
    • Pentraxin-related gene rapidly induced by IL-1 beta antibody
    • Pentraxin-related protein PTX3 antibody
    • PTX3 antibody
    • PTX3_HUMAN antibody
    • TNF alpha-induced protein 5 antibody
    • TNFAIP5 antibody
    • TSG-14 antibody
    • TSG14 antibody
    • Tumor necrosis factor alpha-induced protein 5 antibody
    • Tumor necrosis factor-inducible gene 14 protein antibody
    • Tumor necrosis factor-inducible protein TSG-14 antibody
    see all

Images

  • Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
    Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
    All lanes : Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806) at 1/1000 dilution

    Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell) whole cell lysate
    Lane 2 : HUVEC treated with 0.5ug/ml Lipopolysaccharides(LPS) for 4 hours and then added 300 ng/ml Brefeldin A for another 20 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 41 kDa


    Exposure time: 15 seconds


    The expression profile observed is consistent with what has been described in the literature (PMID:17389238).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
    Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse placenta tissue labeling Pentraxin 3/PTX3 with ab90806 at 1/20 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on mouse placenta was observed. Nuclear counterstain is DAPI (Blue).
    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
    Western blot - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
    All lanes : Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806) at 1/1000 dilution

    Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : Mouse placenta tissue lysate
    Lane 3 : Rat placenta tissue lysate
    Lane 4 : 3T3-L1 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 41 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2: 15 seconds; Lane 3: 3 seconds; Lane 4: 180 seconds.

  • Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)
    Immunohistochemistry (Frozen sections) - Anti-Pentraxin 3/PTX3 antibody [MNB1] (ab90806)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen rat placenta tissue labeling Pentraxin 3/PTX3 with ab90806 at 1/20 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on rat placenta was observed. Nuclear counterstain is DAPI (Blue).
    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab90806? Please let us know so that we can cite the reference in this datasheet.

ab90806 has been referenced in 10 publications.

  • Miyazaki Y  et al. Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model. Sci Rep 11:4690 (2021). PubMed: 33633222
  • Naito T  et al. Mesenchymal stem cells induce tumor stroma formation and epithelial-mesenchymal transition through SPARC expression in colorectal cancer. Oncol Rep 45:N/A (2021). PubMed: 33907853
  • Chang X  et al. Pentraxin 3 is a diagnostic and prognostic marker for ovarian epithelial cancer patients based on comprehensive bioinformatics and experiments. Cancer Cell Int 21:193 (2021). PubMed: 33952272
  • Kendal AR  et al. Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon. Sci Rep 10:13939 (2020). PubMed: 32883960
  • Wu XY  et al. Complement C1q synergizes with PTX3 in promoting NLRP3 inflammasome over-activation and pyroptosis in rheumatoid arthritis. J Autoimmun 106:102336 (2020). PubMed: 31601476
  • Bendícková K  et al. Calcineurin inhibitors reduce NFAT-dependent expression of antifungal pentraxin-3 by human monocytes. J Leukoc Biol N/A:N/A (2019). PubMed: 30934147
  • Scimeca M  et al. Impairment of PTX3 expression in osteoblasts: a key element for osteoporosis. Cell Death Dis 8:e3125 (2017). WB, IHC, ICC/IF ; Human . PubMed: 29022895
  • Mayer C  et al. Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells. Sci Rep 6:37128 (2016). WB ; Human . PubMed: 27849015
  • Belone Ade F  et al. Genome-Wide Screening of mRNA Expression in Leprosy Patients. Front Genet 6:334 (2015). IHC ; Human . PubMed: 26635870
  • Foo SS  et al. Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation. PLoS Pathog 11:e1004649 (2015). PubMed: 25695775

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