Anti-Pepsin antibody (ab181876)
Key features and details
- Goat polyclonal to Pepsin
- Suitable for: ELISA, IHC-P, WB
- Reacts with: Pig
- Isotype: IgG
Overview
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Product name
Anti-Pepsin antibody
See all Pepsin primary antibodies -
Description
Goat polyclonal to Pepsin -
Host species
Goat -
Tested applications
Suitable for: ELISA, IHC-P, WBmore details -
Species reactivity
Reacts with: Pig -
Immunogen
Full length native protein (purified) corresponding to Pig Pepsin aa 1-385. Pepsin protein isolated from pig stomach.
Database link: P00791 -
Positive control
- Pepsin protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.27% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
Anti-PEPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/5000 - 1/20000. Predicted molecular weight: 41 kDa.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/5000 - 1/20000. Predicted molecular weight: 41 kDa. |
Target
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Relevance
Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 396892 Pig
- SwissProt: P00791 Pig
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Alternative names
- Pepsin A 3 antibody
- Pepsin A 4 antibody
- Pepsin A 5 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab181876 has not yet been referenced specifically in any publications.