Product nameAnti-Pepsin antibody (HRP)
See all Pepsin primary antibodies
DescriptionGoat polyclonal to Pepsin (HRP)
Tested applicationsSuitable for: WB, ELISA, Dot blot, Electron Microscopymore details
Species reactivityReacts with: Goat, Pig
Pepsin from Porcine Stomach
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
BSA is IgG and Protease free
Concentration information loading...
Purification notesThis product is purified by a multi-step process including delipidation, salt fractionation, ion exchange chromatography and extensive dialysis against buffer.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab34531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/10000 - 1/50000.
This product has been assayed against 1.0µg of Porcine Pepsin in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.
Suitable for other peroxidase-antibody based enzymatic assays.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevancePepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.
- Pepsin A 3 antibody
- Pepsin A 4 antibody
- Pepsin A 5 antibody
ab34531 has not yet been referenced specifically in any publications.