Product namePepsin Solution (Antigen Retrieval)
Tested applicationsSuitable for: IHC-Pmore details
Pepsin for protease-induced antigen retrieval (PIER) during IHC procedures (ab64201). Ready-to-use formulation. Tris buffer pH 2.0
- Incubate tissue section for 10 minutes at 37°C.
- Rinse and proceed with immunostaining.
Pepsin is a commonly used digestive enzyme for immunohistochemical procedures.
Other products for IHC
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Storage instructionsStore at +4°C.
Storage bufferpH: 2.00
Preservative: 0.1% Sodium azide
Constituents: Tris buffer, 0.25% Pepsin
Concentration information loading...
Reagent notesPepsin is a commonly used digestive enzyme for immunohistochemical procedures.
FunctionShows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Sequence similaritiesBelongs to the peptidase A1 family.
- Information by UniProt
- Pepsin A
- Pepsinogen A5
Our Abpromise guarantee covers the use of ab64201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab64201 has not yet been referenced specifically in any publications.