- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Pepsinogen I antibody [7G3]
See all Pepsinogen I primary antibodies -
DescriptionMouse monoclonal [7G3] to Pepsinogen I
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Host speciesMouse
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Tested applicationsSuitable for: IHC-P, ELISA, Sandwich ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
Full length native protein (purified)
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General notes
This product was changed from ascites to tissue culture supernatant on 11th September, 2017. Lot numbers including and higher than GR3182440-1, GR3182440-2, and GR3182440-3 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.04% PBS, 0.87% Sodium chloride -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number7G3
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab50123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ELISA | Use a concentration of 1 - 10 µg/ml. | |
| Sandwich ELISA | Use at an assay dependent concentration. |
Target
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FunctionShows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
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Sequence similaritiesBelongs to the peptidase A1 family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 643834 Human
- Omim: 169710 Human
- SwissProt: P0DJD8 Human
- Unigene: 601055 Human
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Alternative names
- PEPA3_HUMAN antibody
- Pepsin A-3 antibody
- Pepsinogen 3 group I antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen I antibody [7G3] (ab50123)](https://www.abcam.com/ps/products/50/ab50123/Images/Pepsinogen-I-Primary-antibodies-ab50123-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen I antibody [7G3] (ab50123)IHC image of ab50123 staining in human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50123, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References
ab50123 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"