Anti-Pepsinogen I antibody [7G3] (ab50123)

Reviews (3)Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen I antibody [7G3] (ab50123)

    Key features and details

    • Mouse monoclonal [7G3] to Pepsinogen I
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-Pepsinogen I antibody [7G3]

    • Description

      Mouse monoclonal [7G3] to Pepsinogen I

    • Host species

      Mouse

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Full length native protein (purified)

    • General notes

      This product was changed from ascites to tissue culture supernatant on 11th September, 2017. Lot numbers including and higher than GR3182440-1, GR3182440-2, and GR3182440-3 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.04% PBS, 0.87% Sodium chloride
    • Concentration information loading...

    • Purity

      Protein A purified

    • Clonality

      Monoclonal

    • Clone number

      7G3

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab50123 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P (2)
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Relevance

      Pepsinogen I is proenzyme that is cleaved to form pepsin both in an autocatalytic fashion and by pepsin itself.

    • Alternative names

      • Pepsinogen 1 antibody
      • Pepsinogen A antibody
      • Pepsinogen antibody
      • Pepsinogen-1 antibody
      • Pepsinogen-A antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen I antibody [7G3] (ab50123)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pepsinogen I antibody [7G3] (ab50123)
      IHC image of ab50123 staining in human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50123, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (1)

    Publishing research using ab50123? Please let us know so that we can cite the reference in this datasheet.

    ab50123 has been referenced in 1 publication.

    • Lizárraga-Verdugo E  et al. Cell Survival Is Regulated via SOX9/BCL2L1 Axis in HCT-116 Colorectal Cancer Cell Line. J Oncol 2020:5701527 (2020). PubMed: 32411238

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Pepsinogen I antibody [7G3]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Intestine
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 03 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-Pepsinogen I antibody [7G3]

     Average
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Kidney)
    Specification
    Kidney
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 20 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Pepsinogen I antibody [7G3]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Stomach)
    Specification
    Stomach
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 12 2012

    Question

    Dear Technical,   Our customer would also like to know if they can use one of these to conjugate to Biotin or HRP for colorimetric detection purpose?   I hope to hear from you soon. Kind regards     Dear Technical,   Good day!   We have a customer who is interested in the above antibodies to use as an ELISA pair. But as both these antibodies are mouse raised , the secondary antibody detection will pick up both the captor and the detector antibody. They want to know what is the suggested method of detection?   I hope to hear from you soon.   Kind regards  

    Read More
    Answer

    Thank you for your enquiry. I am sorry it has taken some time to confirm this information. I can confirm that for sandwich ELISA the following have been used for testing: Capture antibody : ab50607 Anti-Pepsinogen I antibody 2F5 Detection antibody : ab50123 Anti-Pepsinogen I antibody 7G3 Regarding conjugation, I can recommend to try our HRP conjugation kit. The conjugation and experiment results woudl then be covered by the Abpomise guarantee: I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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