Overview

  • Product name
    Anti-Pepsinogen II/PGC antibody
    See all Pepsinogen II/PGC primary antibodies
  • Description
    Rabbit polyclonal to Pepsinogen II/PGC
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Baboon, Macaque monkey, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Pepsinogen II/PGC aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab104933)

  • Positive control
    • This antibody gave a positive signal in Human Stomach Tumour Tissue lysate.
  • General notes

     This product was previously labelled as Pepsinogen II

     

Properties

Applications

Our Abpromise guarantee covers the use of ab104595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa).

Target

Images

  • Anti-Pepsinogen II/PGC antibody (ab104595) at 1 µg/ml + Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678) at 10 µg

    Secondary
    Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Pepsinogen II/PGC has a predicted molecular weight of 42-kDa. The first 16 amino acids of the Pepsinogen II/PGC sequence act as a signal sequence. This may explain the migration of the protein at a lower MW than expected.

References

This product has been referenced in:
  • Bertaux-Skeirik N  et al. CD44 plays a functional role in Helicobacter pylori-induced epithelial cell proliferation. PLoS Pathog 11:e1004663 (2015). Flow Cyt . Read more (PubMed: 25658601) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab104595.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up