Overview

  • Product name

    Pepsin/Pepsinogen Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma, Other biological fluids
  • Product overview

    Pepsin/Pepsinogen Assay Kit (Fluorometric) (ab238722) is a homogenous assay that allows for quantification of pepsin activity in gastric tissues (stomach, duodenum etc.) and various biological fluids (serum/plasma, gastric juice, vomit). The assay utilizes a synthetic peptide substrate bearing both a fluorophore and a fluorescence quencher. Upon cleavage by pepsin, the fluorophore-bearing peptide fragment is unquenched to produce a bright fluorescent signal (Ex/Em = 328/418 nm). Lysosomal aspartic proteases in the peptidase A1 family (Cathepsin D and E) do not interfere with the assay. The assay is rapid, simple to perform and is vastly more sensitive than the classical hemoglobin degradation assay, with a detection limit of 500 μU pepsin activity per well.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Mca Standard (1mM) Yellow 1 x 25µl
    Pepsin Assay Buffer 1 x 25ml
    Pepsin Inhibitor (Pepstatin A) 1 x 20µl
    Pepsin Positive Control 1 vial
    Pepsin Substrate 1 x 200µl
  • Research areas

  • Relevance

    Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.
  • Cellular localization

    Secreted
  • Alternative names

    • Pepsin
    • Pepsin A
    • Pepsin A 3
    • Pepsin A 4
    • Pepsin A 5
    • Pepsinogen
    • Pepsinogen 3
    • Pepsinogen 4
    • Pepsinogen 5
    • PGA
    • PGA3
    • PGA4
    • PGA5
    see all

Images

  • One mole of MCA corresponds to the cleavage of one mole of fluorogenic Pepsin Substrate

  • The acid-activated proteases Cathepsin D and E exhibit minimal assay interference, even when present at ≥300-fold excess by mass.

  • Data are mean ± SEM of 3 replicates, assayed according to the kit protocol.

Protocols

References

ab239722 has not yet been referenced specifically in any publications.

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