Overview

  • Product name
    Anti-Peptide YY / PYY antibody
    See all Peptide YY / PYY primary antibodies
  • Description
    Rabbit polyclonal to Peptide YY / PYY
  • Host species
    Rabbit
  • Specificity
    Immunostaining may be abolished by preincubation with 1nmol synthetic porcine PYY per mL diluted antibody. NPY and PP (10nmol/mL) have no effect on immunostaining.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IHC-Rmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Pig
    Predicted to work with: Rabbit, Dog, Human
  • Immunogen

    Full length native protein (purified) corresponding to Pig Peptide YY/ PYY aa 1-36.
    Sequence:

    YPAKPEAPGE DASPEELSRY YASLRHYLNL VTRQRY


    Database link: P68005

  • Positive control
    • Mammalian colonic endocrine (L/EG) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab22663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 11 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr 1/1000 - 1/5000. (Peroxidase-anti-peroxidase procedure). 1/400-1/1600 using indirect immunofluorescence procedure.
IHC-P Use at an assay dependent concentration.
IHC-R 1/1000 - 1/5000. (Peroxidase-anti-peroxidase procedure). 1/400-1/1600 using indirect immunofluorescence procedure.

Target

Images

  • IHC image of Peptide YY / PYY staining in mouse colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • IHC image of Peptide YY / PYY staining in rat pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • Adjacent de-waxed sections of Bouin-fixed fetal (14 day) rat pancreas stained with rabbit polyclonal antisera to PYY (ab22663; panel a) and glucagon (ab18461; panel b). Arrows indicate identical cell profiles. From Ali-Rachedi et al, 1984.
  • ab22663 staining mouse small intestine cells by ICC/IF.  The cells were fixed in acetone, permeabilized in 0.1% TX-100 in AB-solution, and blocked with 10% serum for 30 minutes at 25°C.  The primary antibody was diluted 1/400 in 10% normal goat serum in PBS, 0.1%TX100 and incubated for 24 hours at 4°C.  A FITC conjugated goat anti-rabbit was used as the secondary.

    Blue - DAPI
    Green - Peptide YY / PYY

    See Abreview

  • ab22663 staining Peptide YY / PYY (green) in murine pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue (left, WT; right, Peptide YY / PYY KO) was fixed with paraformaldehyde and blocked with 3% H2O2 in methanol, followed by antigen retrieval with trypsin. Samples were incubated with primary antibody (1/2000 in TNT) overnight. An AlexaFluor®488-conjugated donkey anti-rabbit IgG (1/1000 in TNT) was used as the secondary antibody. Nuclei were stained with DAPI (red).

References

This product has been referenced in:
  • Guida C  et al. Sitagliptin and Roux-en-Y gastric bypass modulate insulin secretion via regulation of intra-islet PYY. Diabetes Obes Metab 20:571-581 (2018). Read more (PubMed: 28892258) »
  • Brooks L  et al. Fermentable carbohydrate stimulates FFAR2-dependent colonic PYY cell expansion to increase satiety. Mol Metab 6:48-60 (2017). Read more (PubMed: 28123937) »

See all 7 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 16 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER
Permeabilization
No
Specification
Pancreas
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 16 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Jejunum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 13 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Pancreas
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Dr. Srividya Vasu

Verified customer

Submitted Oct 08 2013

Thank you for your reply. After following up with the lab, unfortunately we will not be able to add the immunogenic peptide for ab22663 to our catalog. It is possible that you may be able to have the peptide custom synthesized from an outside company u...

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DISCOUNT CODE: ***
Expiration date: August 24th, 2012

I am very pleased to hear you would like to accept our offer and test ab22663 in human. This code will give you: 1 free primary antibody before the expiration date. To redee...

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I am sorry this product did not contain the expected volume and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab22663. To check the status of the order please contact our Customer Servic...

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Thank you for your enquiry. As we discussed, please forward along a representative image of your IHC staining, along with brief descriptions of sample preparation and protocols used. Thanks in advance for the additional information!

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (small intestine)
Permeabilization
Yes - 0.1% TX-100 in AB-solution
Specification
small intestine
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
Username

Dr. Andreas Gille

Verified customer

Submitted Dec 05 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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