Overview

  • Product name

    Anti-Peptide YY/PYY antibody [1] (Biotin)
    See all Peptide YY/PYY primary antibodies
  • Description

    Mouse monoclonal [1] to Peptide YY/PYY (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Specificity

    ab121070 binds free Peptide YY/PYY in solution. Cross-reactivity with NPY or PP in solution is <0.1%.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Peptide YY/ PYY.
    Database link: P10082

  • General notes

    Previously labelled as Peptide YY. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 99% Phosphate Buffer, 0.82% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    1
  • Myeloma

    Sp2
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. as detection antibody.

Target

  • Function

    This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
  • Sequence similarities

    Belongs to the NPY family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • GHYY antibody
    • MGC19143 antibody
    • Peptide tyrosine tyrosine antibody
    • peptide YY antibody
    • Peptide YY like antibody
    • Peptide YY precursor antibody
    • Peptide YY(3-36) antibody
    • Prepro PYY antibody
    • PYY 1 antibody
    • PYY antibody
    • PYY II antibody
    • PYY-I antibody
    • PYY-II antibody
    • PYY_HUMAN antibody
    • PYY1 antibody
    • RATGHYY antibody
    • Yy antibody
    see all

References

ab121070 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.
I have had a look at the products that we have available in our catalogue and I recommend using the following validated antibody pairs for sandwich ELISA (sELISA):
1. Glucagon Like Peptide-1 (active)
I have found two different antibody pairs that have been validated for use in sELISA:
Capture: ab23472 (www.abcam.com/ab23472) and Detection: ab121080(www.abcam.com/ab121080).
or
Capture: ab26278 (www.abcam.com/ab26278) and Detection: ab121072(www.abcam.com/ab121072).
ab26278 is specific for the amidated C terminus of the peptide and does not react with GLP 1 (7-37). It reacts with the amidated C-terminus of GLP 1 (7-36) amide, GLP-1(9-36) and GLP 1 (1-36) amide.
2. Peptide YY
Capture: 121069 (www.abcam.com/ab121069) and Detection: ab121070(www.abcam.com/ab121070).
3. Human Ghrelin
Capture: 121066 (www.abcam.com/ab121066) and Detection: ab121067(www.abcam.com/ab121067).
Attached is a word document with the 4 “standard curves” from the 4 assays. Please note however that these assays have not been optimized and it is very likely that with optimization, you will be able to retrieve a better assay performance than what has been shown here.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab112474.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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