Overview

  • Product name

    Anti-Peptide YY/PYY antibody
    See all Peptide YY/PYY primary antibodies
  • Description

    Rabbit polyclonal to Peptide YY/PYY
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Common marmoset
    Predicted to work with: Dog, Pig, Macaque monkey, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Rat Peptide YY/PYY aa 1 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: P10631
    (Peptide available as ab156889)

  • Positive control

    • This antibody gave a positive signal in Rat normal Pancreas formalin fixed paraffin embedded tissue section.
  • General notes

    Previously labelled as Peptide YY. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • IHC image of Peptide YY/PYY staining in mouse pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/6000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • IHC image of Peptide YY/PYY staining in Human colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/8000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • IHC image of Peptide YY/PYY staining in marmoset Jejunum formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/5000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • IHC image of Peptide YY/PYY staining in Rat normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131246, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Rodnoi P  et al. Neuropeptide Y expression marks partially differentiated ß cells in mice and humans. JCI Insight 2:N/A (2017). ICC/IF ; Mouse . Read more (PubMed: 28614797) »
  • Suzuki H & Yamamoto T Amylin-like immunoreactivity in pancreatic X cells of the black-spotted frog Rana (Pelophylax) nigromaculata. Tissue Cell 46:535-9 (2014). IHC-Fr ; Frog . Read more (PubMed: 25458814) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Pancreas
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Dec 16 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Pancreas
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Dec 13 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
(agent) for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Dec 13 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Jejunum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Dec 12 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up