Overview

  • Product name

    Anti-Peptide YY/PYY antibody
    See all Peptide YY/PYY primary antibodies
  • Description

    Rabbit polyclonal to Peptide YY/PYY
  • Host species

    Rabbit
  • Specificity

    Immunostaining may be abolished by preincubation with 1nmol synthetic porcine PYY per mL diluted antibody. NPY and PP (10nmol/mL) have no effect on immunostaining.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IHC-Rmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Pig
    Predicted to work with: Rabbit, Dog, Human
  • Immunogen

    Full length native protein (purified) corresponding to Pig Peptide YY/PYY aa 1-36.
    Sequence:

    YPAKPEAPGE DASPEELSRY YASLRHYLNL VTRQRY


    Database link: P68005

  • Positive control

    • Mammalian colonic endocrine (L/EG) cells.
  • General notes

    Previously labelled as Peptide YY. 

Properties

Applications

Our Abpromise guarantee covers the use of ab22663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 11 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr 1/1000 - 1/5000. (Peroxidase-anti-peroxidase procedure). 1/400-1/1600 using indirect immunofluorescence procedure.
IHC-P Use at an assay dependent concentration.
IHC-R 1/1000 - 1/5000. (Peroxidase-anti-peroxidase procedure). 1/400-1/1600 using indirect immunofluorescence procedure.

Target

Images

  • IHC image of Peptide YY/PYY staining in mouse colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • IHC image of Peptide YY/PYY staining in rat pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • Adjacent de-waxed sections of Bouin-fixed fetal (14 day) rat pancreas stained with rabbit polyclonal antisera to PYY (ab22663; panel a) and glucagon (ab18461; panel b). Arrows indicate identical cell profiles. From Ali-Rachedi et al, 1984.
  • ab22663 staining mouse small intestine cells by ICC/IF.  The cells were fixed in acetone, permeabilized in 0.1% TX-100 in AB-solution, and blocked with 10% serum for 30 minutes at 25°C.  The primary antibody was diluted 1/400 in 10% normal goat serum in PBS, 0.1%TX100 and incubated for 24 hours at 4°C.  A FITC conjugated goat anti-rabbit was used as the secondary.

    Blue - DAPI
    Green - Peptide YY/PYY

    See Abreview

  • ab22663 staining Peptide YY/PYY (green) in murine pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue (left, WT; right, Peptide YY/PYY KO) was fixed with paraformaldehyde and blocked with 3% H2O2 in methanol, followed by antigen retrieval with trypsin. Samples were incubated with primary antibody (1/2000 in TNT) overnight. An AlexaFluor®488-conjugated donkey anti-rabbit IgG (1/1000 in TNT) was used as the secondary antibody. Nuclei were stained with DAPI (red).

References

This product has been referenced in:

  • Guida C  et al. PYY plays a key role in the resolution of diabetes following bariatric surgery in humans. EBioMedicine 40:67-76 (2019). Read more (PubMed: 30639417) »
  • Lu VB  et al. Adenosine triphosphate is co-secreted with glucagon-like peptide-1 to modulate intestinal enterocytes and afferent neurons. Nat Commun 10:1029 (2019). Read more (PubMed: 30833673) »
See all 12 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Dec 16 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER
Permeabilization
No
Specification
Pancreas
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Dec 16 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Jejunum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Dec 13 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Pancreas
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Srividya Vasu

Verified customer

Submitted Oct 08 2013

Answer

Thank you for your reply. After following up with the lab, unfortunately we will not be able to add the immunogenic peptide for ab22663 to our catalog. It is possible that you may be able to have the peptide custom synthesized from an outside company using the sequence: YPAKPEAPGE DASPEELSRY YASLRHYLNL VTRQRY. I am sorry I could not be more helpful, please let me know if you need any additional information or assistance.

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Question
Answer



DISCOUNT CODE: ***
Expiration date: August 24th, 2012

I am very pleased to hear you would like to accept our offer and test ab22663 in human. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forhuman and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

I am sorry this product did not contain the expected volume and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab22663. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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Answer

Thank you for your enquiry. As we discussed, please forward along a representative image of your IHC staining, along with brief descriptions of sample preparation and protocols used. Thanks in advance for the additional information!

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (small intestine)
Permeabilization
Yes - 0.1% TX-100 in AB-solution
Specification
small intestine
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone

Dr. Andreas Gille

Verified customer

Submitted Dec 05 2007

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