Overview

  • Product name

    Anti-PER1 antibody - N-terminal
    See all PER1 primary antibodies
  • Description

    Rabbit polyclonal to PER1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human PER1 (N terminal). The exact sequence is proprietary.
    Database link: O15534

  • Positive control

    • WB: HEK-293T transfected with PER1, whole cell extract; HEK-293T, A431 and HeLa whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab227872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 136 kDa.

Target

  • Function

    Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
    NPAS2-BMAL1
    BMAL2-induced transactivation.
  • Tissue specificity

    Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle. Low level in kidney.
  • Sequence similarities

    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Post-translational
    modifications

    Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated.
  • Cellular localization

    Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
  • Information by UniProt
  • Database links

  • Alternative names

    • Circadian clock protein PERIOD 1 antibody
    • Circadian clock protein PERIOD1 antibody
    • Circadian pacemaker protein Rigui antibody
    • hPER 1 antibody
    • hPER antibody
    • hPER1 antibody
    • KIAA0482 antibody
    • MGC88021 antibody
    • PER 1 antibody
    • PER antibody
    • PER1 antibody
    • PER1 protein antibody
    • PER1_HUMAN antibody
    • Period 1 antibody
    • Period circadian protein homolog 1 antibody
    • Period drosophila homolog of antibody
    • Period homolog 1 antibody
    • Period1 antibody
    • RIGUI antibody
    see all

Images

  • All lanes : Anti-PER1 antibody - N-terminal (ab227872) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with PER1, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 136 kDa



    5% SDS-PAGE gel.

  • All lanes : Anti-PER1 antibody - N-terminal (ab227872) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 136 kDa



    5% SDS-PAGE gel.

References

ab227872 has not yet been referenced specifically in any publications.

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