Overview

  • Product name

    Anti-PER2 antibody [EPR11381(2)]
    See all PER2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11381(2)] to PER2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PER2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O15055

  • Positive control

    • WB: A673, Y79, HeLa and BxPC-3 cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/10000. Predicted molecular weight: 137 kDa.
ICC/IF 1/50 - 1/200.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
      NPAS2-BMAL1
      BMAL2-induced transactivation.
    • Tissue specificity

      Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung.
    • Involvement in disease

      Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
    • Sequence similarities

      Contains 1 PAC (PAS-associated C-terminal) domain.
      Contains 2 PAS (PER-ARNT-SIM) domains.
    • Post-translational
      modifications

      Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation.
    • Cellular localization

      Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
    • Information by UniProt
    • Database links

    • Alternative names

      • Circadian clock protein PERIOD 2 antibody
      • FASPS antibody
      • FASPS1 antibody
      • hPER 2 antibody
      • hPER2 antibody
      • KIAA0347 antibody
      • OTTHUMP00000164476 antibody
      • PER 2 antibody
      • PER2 antibody
      • PER2_HUMAN antibody
      • Period 2 antibody
      • Period 2 isoform 1 antibody
      • Period circadian clock 2 antibody
      • Period circadian protein 2 antibody
      • Period circadian protein homolog 2 antibody
      • Period homolog 2 (Drosophila) antibody
      • Period homolog 2 antibody
      • Period, Drosophila, homolog of, 2 antibody
      • Period2 antibody
      see all

    Images

    • All lanes : Anti-PER2 antibody [EPR11381(2)] (ab179813) at 1/5000 dilution (purified)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : A673 whole cell lysate
      Lane 3 : BxPC-3 whole cell lysate
      Lane 4 : Y79 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 137 kDa
      Observed band size: 140 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.
    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Flow Cytometry analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • All lanes : Anti-PER2 antibody [EPR11381(2)] (ab179813) at 1/1000 dilution (unpurified)

      Lane 1 : A673 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 137 kDa

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling PER2 with unpurified ab179813 at a dilution of 1/50.

    • Flow cytometric analysis of permeabilized HeLa cells labeling PER2 with unpurified ab179813 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Xiang R  et al. Circadian clock gene Per2 downregulation in non-small cell lung cancer is associated with tumour progression and metastasis. Oncol Rep 40:3040-3048 (2018). Read more (PubMed: 30226549) »
    • Zhang Y  et al. Dosing time dependent in vitro pharmacodynamics of Everolimus despite a defective circadian clock. Cell Cycle 17:33-42 (2018). Read more (PubMed: 29099263) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (chondrocytes cell line)
    Permeabilization
    Yes - TritonX100
    Specification
    chondrocytes cell line
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 0.1% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 03 2018

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