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RecombinantRabMAb

Recombinant Anti-PER2 antibody [EPR11381(2)] (ab179813)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (5)

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Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
  • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)
  • Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)
  • Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
  • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)
  • Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)
  • Anti-PER2 antibody [EPR11381(2)] (ab179813)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11381(2)] to PER2
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human

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Protein
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Recombinant Human PER2 protein (ab112382)
Primary
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Anti-NR1D1 antibody [EPR10376] (ab174309)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PER2 antibody [EPR11381(2)]
    See all PER2 primary antibodies
  • Description

    Rabbit monoclonal [EPR11381(2)] to PER2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PER2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O15055

  • Positive control

    • WB: A673, Y79, HeLa and BxPC-3 cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11381(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Circadian Rhythm
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-PER2 antibody [EPR11381(2)] (ab210652)
    • Alexa Fluor® 594 Anti-PER2 antibody [EPR11381(2)] (ab217086)
    • Anti-PER2 antibody [EPR11381(2)] - BSA and Azide free (ab238973)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human PER2 protein (ab112382)
  • Related Products

    • Recombinant Human PER2 protein (ab112382)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/10 - 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 137 kDa.
ICC/IF (1)
1/50 - 1/200.
Notes
Flow Cyt
1/10 - 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 137 kDa.
ICC/IF
1/50 - 1/200.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
      NPAS2-BMAL1
      BMAL2-induced transactivation.
    • Tissue specificity

      Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung.
    • Involvement in disease

      Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.
    • Sequence similarities

      Contains 1 PAC (PAS-associated C-terminal) domain.
      Contains 2 PAS (PER-ARNT-SIM) domains.
    • Post-translational
      modifications

      Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation.
    • Cellular localization

      Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
    • Target information above from: UniProt accession O15055 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8864 Human
      • Omim: 603426 Human
      • SwissProt: O15055 Human
      • Unigene: 58756 Human
      • Alternative names

        • Circadian clock protein PERIOD 2 antibody
        • FASPS antibody
        • FASPS1 antibody
        • hPER 2 antibody
        • hPER2 antibody
        • KIAA0347 antibody
        • OTTHUMP00000164476 antibody
        • PER 2 antibody
        • PER2 antibody
        • PER2_HUMAN antibody
        • Period 2 antibody
        • Period 2 isoform 1 antibody
        • Period circadian clock 2 antibody
        • Period circadian protein 2 antibody
        • Period circadian protein homolog 2 antibody
        • Period homolog 2 (Drosophila) antibody
        • Period homolog 2 antibody
        • Period, Drosophila, homolog of, 2 antibody
        • Period2 antibody
        see all

      Images

      • Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        All lanes : Anti-PER2 antibody [EPR11381(2)] (ab179813) at 1/5000 dilution (purified)

        Lane 1 : HeLa whole cell lysate
        Lane 2 : A673 whole cell lysate
        Lane 3 : BxPC-3 whole cell lysate
        Lane 4 : Y79 whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 137 kDa
        Observed band size: 140 kDa why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.
      • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)

        Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

        Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

        Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

      • Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)

        Flow Cytometry analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

      • Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        All lanes : Anti-PER2 antibody [EPR11381(2)] (ab179813) at 1/1000 dilution (unpurified)

        Lane 1 : A673 cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : BxPC-3 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 137 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)

        Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling PER2 with unpurified ab179813 at a dilution of 1/50.

      • Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)

        Flow cytometric analysis of permeabilized HeLa cells labeling PER2 with unpurified ab179813 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

      • Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Anti-PER2 antibody [EPR11381(2)] (ab179813)

      Protocols

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (5)

      Publishing research using ab179813? Please let us know so that we can cite the reference in this datasheet.

      ab179813 has been referenced in 5 publications.

      • Xiang R  et al. Circadian clock gene Per2 downregulation in non-small cell lung cancer is associated with tumour progression and metastasis. Oncol Rep 40:3040-3048 (2018). PubMed: 30226549
      • Zhang Y  et al. Dosing time dependent in vitro pharmacodynamics of Everolimus despite a defective circadian clock. Cell Cycle 17:33-42 (2018). PubMed: 29099263
      • Jiang X  et al. Neurodegeneration-associated FUS is a novel regulator of circadian gene expression. Transl Neurodegener 7:24 (2018). PubMed: 30338063
      • Chen B  et al. Per2 participates in AKT-mediated drug resistance in A549/DDP lung adenocarcinoma cells. Oncol Lett 13:423-428 (2017). PubMed: 28123577
      • Muter J  et al. The clock protein period 2 synchronizes mitotic expansion and decidual transformation of human endometrial stromal cells. FASEB J 29:1603-14 (2015). WB . PubMed: 25573754

      Customer reviews and Q&As

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-PER2 antibody [EPR11381(2)]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (chondrocytes cell line)
      Permeabilization
      Yes - TritonX100
      Specification
      chondrocytes cell line
      Blocking step
      BSA as blocking agent for 15 minute(s) · Concentration: 0.1% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 03 2018

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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