Recombinant
RabMAb

Recombinant Anti-PER2 antibody [EPR11381(2)] (ab179813)

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Overview

  • Product name

    Anti-PER2 antibody [EPR11381(2)]
    See all PER2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11381(2)] to PER2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human PER2 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O15055

  • Positive control

    • WB: A673, Y79, HeLa and BxPC-3 cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab179813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Predicted molecular weight: 137 kDa.
ICC/IF 1/50 - 1/200.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
      NPAS2-BMAL1
      BMAL2-induced transactivation.

    • Tissue specificity

      Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung.

    • Involvement in disease

      Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.

    • Sequence similarities

      Contains 1 PAC (PAS-associated C-terminal) domain.
      Contains 2 PAS (PER-ARNT-SIM) domains.

    • Post-translational
      modifications

      Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation.

    • Cellular localization

      Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
    • Information by UniProt

    • Database links

      • Alternative names

        • Circadian clock protein PERIOD 2 antibody
        • FASPS antibody
        • FASPS1 antibody
        • hPER 2 antibody
        • hPER2 antibody
        • KIAA0347 antibody
        • OTTHUMP00000164476 antibody
        • PER 2 antibody
        • PER2 antibody
        • PER2_HUMAN antibody
        • Period 2 antibody
        • Period 2 isoform 1 antibody
        • Period circadian clock 2 antibody
        • Period circadian protein 2 antibody
        • Period circadian protein homolog 2 antibody
        • Period homolog 2 (Drosophila) antibody
        • Period homolog 2 antibody
        • Period, Drosophila, homolog of, 2 antibody
        • Period2 antibody
        see all

      Images

      • Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        All lanes : Anti-PER2 antibody [EPR11381(2)] (ab179813) at 1/5000 dilution (purified)

        Lane 1 : HeLa whole cell lysate
        Lane 2 : A673 whole cell lysate
        Lane 3 : BxPC-3 whole cell lysate
        Lane 4 : Y79 whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 137 kDa
        Observed band size: 140 kDa
        why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.
      • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)

        Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

        Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

        Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

      • Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)

        Flow Cytometry analysis of HeLa cells labelling PER2 with purified ab179813 at a dilution of 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

      • Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Western blot - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        All lanes : Anti-PER2 antibody [EPR11381(2)] (ab179813) at 1/1000 dilution (unpurified)

        Lane 1 : A673 cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : BxPC-3 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 137 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Immunocytochemistry/ Immunofluorescence - Anti-PER2 antibody [EPR11381(2)] (ab179813)

        Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling PER2 with unpurified ab179813 at a dilution of 1/50.

      • Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)
        Flow Cytometry - Anti-PER2 antibody [EPR11381(2)] (ab179813)

        Flow cytometric analysis of permeabilized HeLa cells labeling PER2 with unpurified ab179813 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

      References

      This product has been referenced in:

      • Xiang R  et al. Circadian clock gene Per2 downregulation in non-small cell lung cancer is associated with tumour progression and metastasis. Oncol Rep 40:3040-3048 (2018). Read more (PubMed: 30226549) »
      • Zhang Y  et al. Dosing time dependent in vitro pharmacodynamics of Everolimus despite a defective circadian clock. Cell Cycle 17:33-42 (2018). Read more (PubMed: 29099263) »
      See all 5 Publications for this product

      Publishing research using ab179813? Please let us know so that we can cite the reference in this datasheet.

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-PER2 antibody [EPR11381(2)]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (chondrocytes cell line)
      Permeabilization
      Yes - TritonX100
      Specification
      chondrocytes cell line
      Blocking step
      BSA as blocking agent for 15 minute(s) · Concentration: 0.1% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 03 2018

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